The Bioactive Substance Secreted by MSC Retards Mouse Aortic Vascular Smooth Muscle Cells Calcification

Overview

Journal Biomed Res Int

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
General Medicine

Date 2018 Jul 4

PMID 29967775

Citations 13

Authors

Shuangshuang Wang

Maoqing Tong

Siwang Hu

Xiaomin Chen

Affiliations

Soon will be listed here.

Abstract

Background: Vascular calcification, which is associated with low-level chronic inflammation, is a complication that occurs during aging, atherosclerosis, chronic kidney disease, diabetes mellitus, and hyperlipaemia. In this study, we used conditioned media from mesenchymal stem cells (MSC-CM), a source of autologous cytokines, to test the hypothesis that MSC-CM inhibits vascular smooth muscle cell (VSMC) calcification by suppressing inflammation and apoptosis.

Methods: VSMCs were treated with -glycerophosphate (-GP) to induce calcification and MSC-CM was used as a treatment. Calcium deposition was evaluated using alizarin red and von Kossa staining after a 7-day induction period. Intracellular calcium contents were measured via the o-cresolphthalein complexone method, and alkaline phosphatase (ALP) activity was determined using the para-nitrophenyl phosphate method. The expressions of specific-osteogenic markers, inflammatory cytokines, and apoptosis-associated genes/proteins were examined by real-time polymerase chain reaction or western blotting.

Results: MSC-CM inhibited -GP-induced calcium deposition in VSMCs and decreased intracellular calcium content and ALP activity. Additionally, MSC-CM suppressed the -GP-induced increases in BMP2, Msx2, Runx2, and osteocalcin expression. Additionally, MSC-CM decreased the expression of TNF-, IL-1, and IL-6 in VSMC. MSC-CM also partly blocked -GP-induced VSMC apoptosis, which was associated with an increase in the Bcl-2/Bax expression ratio and a decrease in caspase-3 expression.

Conclusion: Our study results suggest that MSC-CM can inhibit VSMC calcification. This suggests a potential novel clinical application for MSCs in the treatment of vascular calcification and associated diseases.

Citing Articles

Advances in Pathophysiological Mechanisms of Degenerative Aortic Valve Disease.

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Lorentz K, Marini A, Bruk L, Gupta P, Mandal B, DiLeo M Bioengineering (Basel). 2024; 11(9).

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Mesenchymal Stem Cell-conditioned Medium Protecting Renal Tubular Epithelial Cells by Inhibiting Hypoxia-inducible Factor-1α and Nuclear Receptor Coactivator-1.

Liao C, Liu Y, Lin Y, Wang J, Zhou T, Weng W Curr Stem Cell Res Ther. 2023; 19(10):1369-1381.

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Mechanism Analysis of Vascular Calcification Based on Fluid Dynamics.

Xu S, Wang F, Mai P, Peng Y, Shu X, Nie R Diagnostics (Basel). 2023; 13(16).

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