Construction of Cloning, Promoter-screening, and Terminator-screening Shuttle Vectors for Bacillus Subtilis and Streptococcus Lactis

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 1985 Aug 1

PMID 2996425

Citations 43

Authors

J M van der Vossen

J Kok

G Venema

Affiliations

Soon will be listed here.

Abstract

Shuttle vectors have been constructed which are suitable both for the selection of regulatory sequences and for gene cloning in Bacillus subtilis and Streptococcus lactis. The promoter screening vectors contain a promoterless chloramphenicol acetyltransferase gene; the insertion of suitable DNA fragments upstream of the gene restored the enzyme activity. With a related set of vectors, transcription termination signals can be selected.

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