Cancer Cell Death Induced by Ferritins and the Peculiar Role of Their Labile Iron Pool

Overview

Journal Oncotarget

Specialty Oncology

Date 2018 Jul 3

PMID 29963255

Citations 2

Authors

Juan Carlos Cutrin

Diego Alberti

Caterina Bernacchioni

Silvia Ciambellotti

Paola Turano

Claudio Luchinat

Simonetta Geninatti Crich

Silvio Aime

Affiliations

Soon will be listed here.

Abstract

Cellular uptake of human H-ferritin loaded with 50 or 350 iron ions results in significant cytotoxicity on HeLa cells at submicromolar concentrations. Conversely, Horse Spleen Ferritin, that can be considered a model of L-cages, as it contains only about 10% of H subunits, even when loaded with 1000 iron ions, is toxic only at >1 order of magnitude higher protein concentrations. We propose here that the different cytotoxicity of the two ferritin cages originates from the presence in H-ferritin of a pool of non-biomineralized iron ions bound at the ferroxidase catalytic sites of H-ferritin subunits. This iron pool is readily released during the endosomal-mediated H-ferritin internalization.

Citing Articles

SCARA5 induced ferroptosis to effect ESCC proliferation and metastasis by combining with Ferritin light chain.

Liu Y, Xiong R, Xiao T, Xiong L, Wu J, Li J BMC Cancer. 2022; 22(1):1304.

PMID: 36513999 PMC: 9746006. DOI: 10.1186/s12885-022-10414-9.

Biochemistry of mammalian ferritins in the regulation of cellular iron homeostasis and oxidative responses.

Zhang J, Chen X, Hong J, Tang A, Liu Y, Xie N Sci China Life Sci. 2020; 64(3):352-362.

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