Association Between Polymorphisms in Genes Encoding Estrogen Receptors (ESR1 and ESR2) and Excreted Bisphenol A Levels After Orthodontic Bracket Bonding: a Preliminary Study

Overview

Journal Prog Orthod

Publisher Springer

Specialty Dentistry

Date 2018 Jul 3

PMID 29961922

Authors

Karla C Horta

Guido A Maranon-Vasquez

Mirian A N Matsumoto

Marilia R Moreira

Fabio L Romano

Alberto Consolaro

Israel D de Souza

Tamires A V Brigante

Maria E C Queiroz

Paulo Nelson-Filho

Erika C Kuchler

Affiliations

Soon will be listed here.

Abstract

Background: Bisphenol A (BPA) is released from orthodontic composites used for bracket bonding. Genetic variations could modify the metabolism of this chemical within the organism. Considering that free BPA binds to estrogen receptors causing harmful effects to health, the present in vivo study aimed to evaluate the association between genetic polymorphisms in genes encoding estrogen receptors (ESR1 and ESR2) and excreted BPA levels in orthodontic patients.

Methods: Quantification of BPA levels in the urine of 16 patients was performed in a gas chromatograph mass spectrometer before (T0), at 24 h (T1), and 1 week (T2) after bracket bonding. DNA was extracted from saliva, and one genetic polymorphism in ESR1 (rs2234693) and two in ESR2 (rs4986938 and rs1256049) were analyzed by real-time PCR. Increases in BPA levels in the urine at T1 and T2 were grouped according to the genotype, and mean differences were compared by unpaired T test or Mann-Whitney test according to the normality of the data. The established alpha was 5%.

Results: BPA levels increased significantly at T1 and T2. There were no statistically significant differences in the increases in BPA levels according to the genotype for any genetic polymorphism (P > 0.05), at neither 24 h nor 1 week after bracket bonding.

Conclusions: The results suggested that there are no association between excreted BPA levels after bracket bonding and the evaluated genetic polymorphisms in ESR1 and ESR2. Further research should be performed in order to confirm these results.

References

Vandenberg L, Hauser R, Marcus M, Olea N, Welshons W . Human exposure to bisphenol A (BPA). Reprod Toxicol. 2007; 24(2):139-77. DOI: 10.1016/j.reprotox.2007.07.010. View

Partosch F, Mielke H, Gundert-Remy U . Functional UDP-glucuronyltransferase 2B15 polymorphism and bisphenol A concentrations in blood: results from physiologically based kinetic modelling. Arch Toxicol. 2013; 87(7):1257-64. DOI: 10.1007/s00204-013-1022-8. View

Chen L, Fan Y, Kao P, Yin Ho D, Ha J, Chu L . Genetic Polymorphisms in Estrogen Metabolic Pathway Associated with Risks of Alzheimer's Disease: Evidence from a Southern Chinese Population. J Am Geriatr Soc. 2017; 65(2):332-339. DOI: 10.1111/jgs.14537. View

Li G, Yang M, Li X, Deng S . Comprehensive assessment of the association between estrogen receptor of alpha polymorphisms and the risk of prostate cancer: evidence from a meta-analysis. Oncotarget. 2017; 8(60):102310-102320. PMC: 5731956. DOI: 10.18632/oncotarget.21117. View

Kurosawa T, Hiroi H, Tsutsumi O, Ishikawa T, Osuga Y, Fujiwara T . The activity of bisphenol A depends on both the estrogen receptor subtype and the cell type. Endocr J. 2002; 49(4):465-71. DOI: 10.1507/endocrj.49.465. View

Geens T, Goeyens L, Covaci A . Are potential sources for human exposure to bisphenol-A overlooked?. Int J Hyg Environ Health. 2011; 214(5):339-47. DOI: 10.1016/j.ijheh.2011.04.005. View

Eliades T, Hiskia A, Eliades G, Athanasiou A . Assessment of bisphenol-A release from orthodontic adhesives. Am J Orthod Dentofacial Orthop. 2007; 131(1):72-5. DOI: 10.1016/j.ajodo.2006.08.013. View

Papakonstantinou A, Eliades T, Cellesi F, Watts D, Silikas N . Evaluation of UDMA's potential as a substitute for Bis-GMA in orthodontic adhesives. Dent Mater. 2013; 29(8):898-905. DOI: 10.1016/j.dental.2013.05.007. View

Welshons W, Nagel S, Vom Saal F . Large effects from small exposures. III. Endocrine mechanisms mediating effects of bisphenol A at levels of human exposure. Endocrinology. 2006; 147(6 Suppl):S56-69. DOI: 10.1210/en.2005-1159. View

10.

Trasande L, Spanier A, Sathyanarayana S, Attina T, Blustein J . Urinary phthalates and increased insulin resistance in adolescents. Pediatrics. 2013; 132(3):e646-55. PMC: 4528350. DOI: 10.1542/peds.2012-4022. View

11.

Berge T, Lygre G, Jonsson B, Lindh C, Bjorkman L . Bisphenol A concentration in human saliva related to dental polymer-based fillings. Clin Oral Investig. 2017; 21(8):2561-2568. DOI: 10.1007/s00784-017-2055-9. View

12.

. Determination of bisphenol a released from resin-based composite dental restoratives. J Am Dent Assoc. 2014; 145(7):763-5. DOI: 10.14219/jada.2014.42. View

13.

Geens T, Aerts D, Berthot C, Bourguignon J, Goeyens L, Lecomte P . A review of dietary and non-dietary exposure to bisphenol-A. Food Chem Toxicol. 2012; 50(10):3725-40. DOI: 10.1016/j.fct.2012.07.059. View

14.

Floyd C, Dickens S . Network structure of Bis-GMA- and UDMA-based resin systems. Dent Mater. 2005; 22(12):1143-9. DOI: 10.1016/j.dental.2005.10.009. View

15.

Ginsberg G, Rice D . Does rapid metabolism ensure negligible risk from bisphenol A?. Environ Health Perspect. 2010; 117(11):1639-43. PMC: 2801165. DOI: 10.1289/ehp.0901010. View

16.

Street C, Zhu Z, Finel M, Court M . Bisphenol-A glucuronidation in human liver and breast: identification of UDP-glucuronosyltransferases (UGTs) and influence of genetic polymorphisms. Xenobiotica. 2016; 47(1):1-10. PMC: 5531270. DOI: 10.3109/00498254.2016.1156784. View

17.

Li T, Wu Q, Zhang C, Li W, Li N, Cui Y . Polymorphisms in estrogen receptors predict the risk of male infertility: a meta-analysis. Reprod Biol Endocrinol. 2014; 12:79. PMC: 4141121. DOI: 10.1186/1477-7827-12-79. View

18.

Trasande L, Attina T, Trachtman H . Bisphenol A exposure is associated with low-grade urinary albumin excretion in children of the United States. Kidney Int. 2013; 83(4):741-8. PMC: 3709970. DOI: 10.1038/ki.2012.422. View

19.

Akin L, Kendirci M, Narin F, Kurtoglu S, Saraymen R, Kondolot M . The endocrine disruptor bisphenol A may play a role in the aetiopathogenesis of polycystic ovary syndrome in adolescent girls. Acta Paediatr. 2014; 104(4):e171-7. DOI: 10.1111/apa.12885. View

20.

Thayer K, Doerge D, Hunt D, Schurman S, Twaddle N, Churchwell M . Pharmacokinetics of bisphenol A in humans following a single oral administration. Environ Int. 2015; 83:107-15. PMC: 4545316. DOI: 10.1016/j.envint.2015.06.008. View