Single-cell Epigenetics - Chromatin Modification Atlas Unveiled by Mass Cytometry

Overview

Journal Clin Immunol

Specialty Allergy & Immunology

Date 2018 Jul 1

PMID 29960011

Citations 15

Authors

Peggie Cheung

Francesco Vallania

Mai Dvorak

Sarah E Chang

Steven Schaffert

Michele Donato

Aditya M Rao

Rong Mao

Paul J Utz

Purvesh Khatri

Alex J Kuo

Affiliations

Soon will be listed here.

Abstract

Modifications of histone proteins are fundamental to the regulation of epigenetic phenotypes. Dysregulations of histone modifications have been linked to the pathogenesis of diverse human diseases. However, identifying differential histone modifications in patients with immune-mediated diseases has been challenging, in part due to the lack of a powerful analytic platform to study histone modifications in the complex human immune system. We recently developed a highly multiplexed platform, Epigenetic landscape profiling using cytometry by Time-Of-Flight (EpiTOF), to analyze the global levels of a broad array of histone modifications in single cells using mass cytometry. In this review, we summarize the development of EpiTOF and discuss its potential applications in biomedical research. We anticipate that this platform will provide new insights into the roles of epigenetic regulation in hematopoiesis, immune cell functions, and immune system aging, and reveal aberrant epigenetic patterns associated with immune-mediated diseases.

Citing Articles

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