UV-induced Mutagenesis of Phage S13 Can Occur in the Absence of the RecA and UmuC Proteins of Escherichia Coli

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1985 Oct 1

PMID 2995974

Citations 21

Authors

I Tessman

Affiliations

Soon will be listed here.

Abstract

The UV-induced mutagenesis of phage S13 that accompanies Weigle repair is known to require the products of the recA and umuDC genes, as does the UV-induced mutagenesis of the Escherichia coli chromosome. I found that UV-induced mutagenesis of phage S13 occurred in the absence of both the RecA and UmuC functions when the irradiated phage was photoreactivated. Furthermore, UV-induced phage mutations were produced in a recA- umuC- cell even without photoreactivation and in the absence of any other known UV repair mechanism, at a frequency 29% of that found after photoreactivation and 7% of that found after Weigle repair, implying that DNA synthesis can proceed past a dimer at an unexpectedly high frequency even when unaided by the UmuC-RecA SOS repair functions. The unaided DNA synthesis appears capable of producing mutations in the vicinity of a pyrimidine dimer; by aiding synthesis past a dimer, a repair mechanism may disclose a mutation without having any active role in producing it.

Citing Articles

Isolation of a novel Aggregatibacter actinomycetemcomitans serotype b bacteriophage capable of lysing bacteria within a biofilm.

Castillo-Ruiz M, Vines E, Montt C, Fernandez J, Delgado J, Hormazabal J Appl Environ Microbiol. 2011; 77(9):3157-9.

PMID: 21378052 PMC: 3126385. DOI: 10.1128/AEM.02115-10.

Efficient translesion replication in the absence of Escherichia coli Umu proteins and 3'-5' exonuclease proofreading function.

Vandewiele D, Borden A, OGrady P, Woodgate R, Lawrence C Proc Natl Acad Sci U S A. 1998; 95(26):15519-24.

PMID: 9861001 PMC: 28075. DOI: 10.1073/pnas.95.26.15519.

SOS factors involved in translesion synthesis.

Napolitano R, Lambert I, Fuchs R Proc Natl Acad Sci U S A. 1997; 94(11):5733-8.

PMID: 9159142 PMC: 20848. DOI: 10.1073/pnas.94.11.5733.

Reconstitution of repair-gap UV mutagenesis with purified proteins from Escherichia coli: a role for DNA polymerases III and II.

Tomer G, ODonnell M, Goodman M, Livneh Z Proc Natl Acad Sci U S A. 1996; 93(4):1376-80.

PMID: 8643639 PMC: 39945. DOI: 10.1073/pnas.93.4.1376.

On reliability analysis of DNA repair systems.

Joshi R Bull Math Biol. 1987; 49(2):187-216.

PMID: 3607339 DOI: 10.1007/BF02459698.

References

Bagg A, Kenyon C, Walker G . Inducibility of a gene product required for UV and chemical mutagenesis in Escherichia coli. Proc Natl Acad Sci U S A. 1981; 78(9):5749-53. PMC: 348850. DOI: 10.1073/pnas.78.9.5749. View

Blanco M, Herrera G, Collado P, Rebollo J, Botella L . Influence of RecA protein on induced mutagenesis. Biochimie. 1982; 64(8-9):633-6. DOI: 10.1016/s0300-9084(82)80102-8. View

Baker R, DONIGER J, Tessman I . Roles of parental and progeny DNA in 2 mechanisms of phage S13 recombination. Nat New Biol. 1971; 230(1):23-5. DOI: 10.1038/newbio230023a0. View

Sagher D, Strauss B . Insertion of nucleotides opposite apurinic/apyrimidinic sites in deoxyribonucleic acid during in vitro synthesis: uniqueness of adenine nucleotides. Biochemistry. 1983; 22(19):4518-26. DOI: 10.1021/bi00288a026. View

Baas P, Jansz H . Asymmetric information transfer during phi X174 DNA replication. J Mol Biol. 1972; 63(3):557-68. DOI: 10.1016/0022-2836(72)90447-0. View

Wildenberg J, Meselson M . Mismatch repair in heteroduplex DNA. Proc Natl Acad Sci U S A. 1975; 72(6):2202-6. PMC: 432725. DOI: 10.1073/pnas.72.6.2202. View

Dari R, Huisman O . DNA replication and indirect induction of the SOS response in Escherichia coli. Biochimie. 1982; 64(8-9):623-7. DOI: 10.1016/s0300-9084(82)80100-4. View

Tessman I . Genetic recombination of phage S13 in a recombination-deficient mutant of Escherichia coli K12. Biochem Biophys Res Commun. 1966; 22(2):169-74. DOI: 10.1016/0006-291x(66)90427-x. View

Tessman I . Selective stimulation of one of the mechanisms for genetic recombination of bacteriophage S13. Science. 1968; 161(3840):481-2. DOI: 10.1126/science.161.3840.481. View

10.

Weigle J . Induction of Mutations in a Bacterial Virus. Proc Natl Acad Sci U S A. 1953; 39(7):628-36. PMC: 1063835. DOI: 10.1073/pnas.39.7.628. View

11.

Walker G . Mutagenesis and inducible responses to deoxyribonucleic acid damage in Escherichia coli. Microbiol Rev. 1984; 48(1):60-93. PMC: 373003. DOI: 10.1128/mr.48.1.60-93.1984. View

12.

Nevers P, Spatz H . Escherichia coli mutants uvr D and uvr E deficient in gene conversion of lambda-heteroduplexes. Mol Gen Genet. 1975; 139(3):233-43. DOI: 10.1007/BF00268974. View

13.

Howard B, Tessman I . IDENTIFICATION OF THE ALTERED BASES IN MUTATED SINGLE-STRANDED DNA. 3. MUTAGENESIS BY ULTRAVIOLET LIGHT. J Mol Biol. 1964; 9:372-5. DOI: 10.1016/s0022-2836(64)80214-x. View

14.

Elledge S, Walker G . Proteins required for ultraviolet light and chemical mutagenesis. Identification of the products of the umuC locus of Escherichia coli. J Mol Biol. 1983; 164(2):175-92. DOI: 10.1016/0022-2836(83)90074-8. View

15.

Krueger J, Elledge S, Walker G . Isolation and characterization of Tn5 insertion mutations in the lexA gene of Escherichia coli. J Bacteriol. 1983; 153(3):1368-78. PMC: 221787. DOI: 10.1128/jb.153.3.1368-1378.1983. View

16.

Csonka L, Clark A . Deletions generated by the transposon Tn10 in the srl recA region of the Escherichia coli K-12 chromosome. Genetics. 1979; 93(2):321-43. PMC: 1214081. DOI: 10.1093/genetics/93.2.321. View

17.

TESSMAN E, Ozaki T . The interaction of phage S13 with ultraviolet-irradiated host cells and properties of the ultraviolet-irradiated phage. Virology. 1960; 12:431-49. DOI: 10.1016/0042-6822(60)90165-3. View

18.

Lu A, Clark S, Modrich P . Methyl-directed repair of DNA base-pair mismatches in vitro. Proc Natl Acad Sci U S A. 1983; 80(15):4639-43. PMC: 384099. DOI: 10.1073/pnas.80.15.4639. View

19.

Bridges B, Woodgate R . Mutagenic repair in Escherichia coli. X. The umuC gene product may be required for replication past pyrimidine dimers but not for the coding error in UV-mutagenesis. Mol Gen Genet. 1984; 196(2):364-6. DOI: 10.1007/BF00328073. View

20.

Schaaper R, Kunkel T, Loeb L . Infidelity of DNA synthesis associated with bypass of apurinic sites. Proc Natl Acad Sci U S A. 1983; 80(2):487-91. PMC: 393403. DOI: 10.1073/pnas.80.2.487. View