Binding of Protein Kinase C to Neutrophil Membranes in the Presence of Ca2+ and Its Activation by a Ca2+-requiring Proteinase

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1985 Oct 1

PMID 2995965

Citations 35

Authors

E Melloni

S Pontremoli

M Michetti

O Sacco

B Sparatore

F Salamino

B L HORECKER

Affiliations

Soon will be listed here.

Abstract

In the presence of micromolar concentrations of Ca2+, both protein kinase C and a cytosolic Ca2+-requiring neutral proteinase of human neutrophils become associated with the neutrophil membrane. Binding to the membrane results in activation of the proteinase, which then catalyzes limited proteolysis of the kinase to produce a form that is fully active in the absence of Ca2+ and phospholipid. This irreversibly activated protein kinase is released from the membrane and may thus have access, in the intact cell, to intracellular protein substrates. In the absence of the proteinase, Ca2+ promotes the binding of protein kinase C, but conversion to the Ca2+/phospholipid-independent form does not occur and the kinase remains associated with the membrane fraction.

Citing Articles

Ca2+ promotes erythrocyte band 3 tyrosine phosphorylation via dissociation of phosphotyrosine phosphatase from band 3.

Zipser Y, Piade A, Barbul A, Korenstein R, Kosower N Biochem J. 2002; 368(Pt 1):137-44.

PMID: 12175337 PMC: 1222980. DOI: 10.1042/BJ20020359.

Calpain regulates actin remodeling during cell spreading.

Potter D, Tirnauer J, Janssen R, Croall D, Hughes C, Fiacco K J Cell Biol. 1998; 141(3):647-62.

PMID: 9566966 PMC: 2132736. DOI: 10.1083/jcb.141.3.647.

Stimulation of protein kinase C redistribution and inhibition of leukotriene B4-induced inositol 1,4,5-trisphosphate generation in human neutrophils by lipoxin A4.

Soyombo O, Spur B, Lee T Br J Pharmacol. 1996; 117(6):1334-40.

PMID: 8882633 PMC: 1909807. DOI: 10.1111/j.1476-5381.1996.tb16733.x.

Biologically active monomeric and heterodimeric recombinant human calpain I produced using the baculovirus expression system.

Meyer S, Bozyczko-Coyne D, Mallya S, Spais C, Bihovsky R, Kaywooya J Biochem J. 1996; 314 ( Pt 2):511-9.

PMID: 8670065 PMC: 1217080. DOI: 10.1042/bj3140511.

Calcium-dependent protein kinase C activity of neutrophils in localized juvenile periodontitis.

Kurihara H, Murayama Y, Warbington M, Champagne C, Van Dyke T Infect Immun. 1993; 61(8):3137-42.

PMID: 8335344 PMC: 280980. DOI: 10.1128/iai.61.8.3137-3142.1993.

References

Baenziger N, MAJERUS P . Isolation of human platelets and platelet surface membranes. Methods Enzymol. 1974; 31:149-55. DOI: 10.1016/0076-6879(74)31015-4. View

Winterhalter K, Huehns E . PREPARATIONS, PROPERTIES, AND SPECIFIC RECOMBINATION OF ALPHA-BETA-GLOBIN SUBUNITS. J Biol Chem. 1964; 239:3699-705. View

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

Takai Y, Kishimoto A, Inoue M, NISHIZUKA Y . Studies on a cyclic nucleotide-independent protein kinase and its proenzyme in mammalian tissues. I. Purification and characterization of an active enzyme from bovine cerebellum. J Biol Chem. 1977; 252(21):7603-9. View

Inoue M, Kishimoto A, Takai Y, NISHIZUKA Y . Studies on a cyclic nucleotide-independent protein kinase and its proenzyme in mammalian tissues. II. Proenzyme and its activation by calcium-dependent protease from rat brain. J Biol Chem. 1977; 252(21):7610-6. View

DEWALD B, Baggiolini M, Curnutte J, Babior B . Subcellular localization of the superoxide-forming enzyme in human neutrophils. J Clin Invest. 1979; 63(1):21-9. PMC: 371913. DOI: 10.1172/JCI109273. View

Petroski R, Naccache P, Becker E, Shaafi R . Effect of the chemotactic factor formyl methionyl- leucyl-phenylalanine and cytochalasin B on the cellular levels of calcium in rabbit neutrophils. FEBS Lett. 1979; 100(1):161-5. DOI: 10.1016/0014-5793(79)81155-2. View

Takai Y, Kishimoto A, Iwasa Y, Kawahara Y, Mori T, NISHIZUKA Y . Calcium-dependent activation of a multifunctional protein kinase by membrane phospholipids. J Biol Chem. 1979; 254(10):3692-5. View

Hoffstein S . Ultrastructural demonstration of calcium loss from local regions of the plasma membrane of surface-stimulated human granulocytes. J Immunol. 1979; 123(3):1395-402. View

10.

Takai Y, Kishimoto A, Iwasa Y, Kawahara Y, Mori T, NISHIZUKA Y . A role of membranes in the activation of a new multifunctional protein kinase system. J Biochem. 1979; 86(2):575-8. DOI: 10.1093/oxfordjournals.jbchem.a132557. View

11.

Takai Y, Kishimoto A, Kikkawa U, Mori T, NISHIZUKA Y . Unsaturated diacylglycerol as a possible messenger for the activation of calcium-activated, phospholipid-dependent protein kinase system. Biochem Biophys Res Commun. 1979; 91(4):1218-24. DOI: 10.1016/0006-291x(79)91197-5. View

12.

Kishimoto A, Takai Y, Mori T, Kikkawa U, NISHIZUKA Y . Activation of calcium and phospholipid-dependent protein kinase by diacylglycerol, its possible relation to phosphatidylinositol turnover. J Biol Chem. 1980; 255(6):2273-6. View

13.

Katoh N, Wrenn R, Wise B, Shoji M, Kuo J . Substrate proteins for calmodulin-sensitive and phospholipid-sensitive Ca2+-dependent protein kinases in heart, and inhibition of their phosphorylation by palmitoylcarnitine. Proc Natl Acad Sci U S A. 1981; 78(8):4813-7. PMC: 320258. DOI: 10.1073/pnas.78.8.4813. View

14.

Mottola C, Romeo D . Calcium movement and membrane potential changes in the early phase of neutrophil activation by phorbol myristate acetate: a study with ion-selective electrodes. J Cell Biol. 1982; 93(1):129-34. PMC: 2112096. DOI: 10.1083/jcb.93.1.129. View

15.

Katoh N, Kuo J . Subcellular distribution of phospholipid-sensitive calcium-dependent protein kinase in guinea pig heart, spleen and cerebral cortex, and inhibition of the enzyme by Triton X-100. Biochem Biophys Res Commun. 1982; 106(2):590-5. DOI: 10.1016/0006-291x(82)91151-2. View

16.

Kikkawa U, Takai Y, Minakuchi R, Inohara S, NISHIZUKA Y . Calcium-activated, phospholipid-dependent protein kinase from rat brain. Subcellular distribution, purification, and properties. J Biol Chem. 1982; 257(22):13341-8. View

17.

Kishimoto A, Kajikawa N, Shiota M, NISHIZUKA Y . Proteolytic activation of calcium-activated, phospholipid-dependent protein kinase by calcium-dependent neutral protease. J Biol Chem. 1983; 258(2):1156-64. View

18.

Sano K, Takai Y, Yamanishi J, NISHIZUKA Y . A role of calcium-activated phospholipid-dependent protein kinase in human platelet activation. Comparison of thrombin and collagen actions. J Biol Chem. 1983; 258(3):2010-3. View

19.

Helfman D, Appelbaum B, VOGLER W, Kuo J . Phospholipid-sensitive Ca2+-dependent protein kinase and its substrates in human neutrophils. Biochem Biophys Res Commun. 1983; 111(3):847-53. DOI: 10.1016/0006-291x(83)91376-1. View

20.

Wrenn R . Phospholipid-sensitive calcium-dependent protein kinase and its endogenous substrate proteins in rat pancreatic acinar cells. Life Sci. 1983; 32(20):2385-92. DOI: 10.1016/0024-3205(83)90770-1. View