Neuroprotective Effects of Dietary Borax in the Brain Tissue of Rainbow Trout (Oncorhynchus Mykiss) Exposed to Copper-induced Toxicity

Overview

Journal Fish Physiol Biochem

Specialty Biochemistry

Date 2018 Jul 1

PMID 29959587

Citations 3

Authors

Gonca Alak

Arzu Ucar

Asli Cilingir Yeltekin

Selim Comakli

Veysel Parlak

Ismail Hakki Tas

Mustafa Ozkaraca

Ahmet Topal

Esra Manavoglu Kirman

Ismail Bolat

Muhammed Atamanalp

Hasan Turkez

Affiliations

Soon will be listed here.

Abstract

We aimed to investigate the modulating effects of dietary borax on the pathways in rainbow trout brain exposed to copper. For this aim, a comprehensive assessment was performed including biochemical (acetylcholinesterase (AChE), malondialdehyde (MDA), oxidative DNA damage (8-hydroxy-2'-deoxyguanosine (8-OHdG), and caspase-3 levels) and transcriptional parameters (heat shock protein 70 (HSP70) and cytochromes P450 (CYP1A), glutathione peroxidase (gpx), superoxide dismutase (sod), and catalase (cat)) parameters and immunohistochemically staining of 8-OHdG. Special fish feed diets were prepared for the trial. These diets contained different concentrations of borax (1.25, 2.5, and 5 mg/kg) and/or copper (500 and 1000 mg/kg) at the period of pre- and co-treatment strategies for 21 days. At the end of the treatment periods, brain tissue was sampled for each experimental group. As a result, the biochemical parameters were increased and AChE activity decreased in the copper and copper-combined groups in comparison with the control group and also with only borax applications (p < 0.05). We observed an increase or decrease in particular biochemical parameters for the borax group in every application and we established that borax had protective effect against copper toxicity by decreasing and/or increasing the relevant biochemical parameters in brain tissue of fish. The biochemical results of borax and its combinations corresponded to the observations of gene expression data, which similarly concluded that HSP70 and CYP1A genes were strongly induced by copper (p < 0.05). In addition, the expression levels of the sod, cat, and gpx genes in the fish brains exposed to borax and the borax combination groups were significantly higher than the only copper-treated groups. In conclusion, borax supplementation provided significant protection against copper-induced neurotoxicity in trout.

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References

Valdehita A, Fernandez-Cruz M, Torrent F, Sericano J, Navas J . Differences in the induction of cyp1A and related genes in cultured rainbow trout Oncorhynchus mykiss. Additional considerations for the use of EROD activity as a biomarker. J Fish Biol. 2012; 81(1):270-87. DOI: 10.1111/j.1095-8649.2012.03338.x. View

Benhamed S, Guardiola F, Martinez S, Martinez-Sanchez M, Perez-Sirvent C, Mars M . Exposure of the gilthead seabream () to sediments contaminated with heavy metals down-regulates the gene expression of stress biomarkers. Toxicol Rep. 2017; 3:364-372. PMC: 5615830. DOI: 10.1016/j.toxrep.2016.02.006. View

Yu L, Yu H, Li N, Wang X, Li F, Wu X . Dietary butylated hydroxytoluene improves lipid metabolism, antioxidant and anti-apoptotic response of largemouth bass (Micropterus salmoides). Fish Shellfish Immunol. 2017; 72:220-229. DOI: 10.1016/j.fsi.2017.10.054. View

Topal A, Alak G, Altun S, Erol H, Atamanalp M . Evaluation of 8-hydroxy-2-deoxyguanosine and NFkB activation, oxidative stress response, acetylcholinesterase activity, and histopathological changes in rainbow trout brain exposed to linuron. Environ Toxicol Pharmacol. 2016; 49:14-20. DOI: 10.1016/j.etap.2016.11.009. View

Ko J, Gwak M, Kim G, Shin Y, Ryu S, Kim J . The protective effect of ischemic preconditioning against hepatic ischemic-reperfusion injury under isoflurane anesthesia in rats. Transplant Proc. 2013; 45(5):1704-7. DOI: 10.1016/j.transproceed.2012.08.026. View

Rios-Sicairos J, Betancourt-Lozano M, Leal-Tarin B, Hernandez-Cornejo R, Aguilar-Zarate G, Garcia-De-La-Parra L . Heat-shock protein (Hsp70) and cytochrome P-450 (CYP1A) in the white mullet Mugil curema (Pisces:Mugilidae) as biomarkers to assess environmental quality in coastal lagoons. J Environ Sci Health A Tox Hazard Subst Environ Eng. 2010; 45(1):68-74. DOI: 10.1080/10934520903388855. View

Urani C, Melchioretto P, Morazzoni F, Canevali C, Camatini M . Copper and zinc uptake and hsp70 expression in HepG2 cells. Toxicol In Vitro. 2001; 15(4-5):497-502. DOI: 10.1016/s0887-2333(01)00054-6. View

Altun S, Ozdemir S, Arslan H . Histopathological effects, responses of oxidative stress, inflammation, apoptosis biomarkers and alteration of gene expressions related to apoptosis, oxidative stress, and reproductive system in chlorpyrifos-exposed common carp (Cyprinus carpio L.). Environ Pollut. 2017; 230:432-443. DOI: 10.1016/j.envpol.2017.06.085. View

Weydert C, Cullen J . Measurement of superoxide dismutase, catalase and glutathione peroxidase in cultured cells and tissue. Nat Protoc. 2010; 5(1):51-66. PMC: 2830880. DOI: 10.1038/nprot.2009.197. View

10.

Parlak V . Evaluation of apoptosis, oxidative stress responses, AChE activity and body malformations in zebrafish (Danio rerio) embryos exposed to deltamethrin. Chemosphere. 2018; 207:397-403. DOI: 10.1016/j.chemosphere.2018.05.112. View

11.

Tang Y, Han L, Chen X, Xie M, Kong W, Wu Z . Dietary Supplementation of Probiotic Bacillus subtilis Affects Antioxidant Defenses and Immune Response in Grass Carp Under Aeromonas hydrophila Challenge. Probiotics Antimicrob Proteins. 2018; 11(2):545-558. DOI: 10.1007/s12602-018-9409-8. View

12.

Turkez H, Geyikoglu F, Dirican E, Tatar A . In vitro studies on chemoprotective effect of borax against aflatoxin B1-induced genetic damage in human lymphocytes. Cytotechnology. 2012; 64(6):607-12. PMC: 3488367. DOI: 10.1007/s10616-012-9454-1. View

13.

Jeon H, Lee Y, Mo H, Kim M, Al-Wabel M, Kim Y . Chlorpyrifos-induced biomarkers in Japanese medaka (Oryzias latipes). Environ Sci Pollut Res Int. 2015; 23(2):1071-80. DOI: 10.1007/s11356-015-4598-0. View

14.

Uram L, Niziol J, Maj P, Sobich J, Rode W, Ruman T . N(4)-[B-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan)methyl]-2'-deoxycytidine as a potential boron delivery agent with respect to glioblastoma. Biomed Pharmacother. 2017; 95:749-755. DOI: 10.1016/j.biopha.2017.08.134. View

15.

Luo Y, Su Y, Lin R, Shi H, Wang X . 2-chlorophenol induced ROS generation in fish Carassius auratus based on the EPR method. Chemosphere. 2006; 65(6):1064-73. DOI: 10.1016/j.chemosphere.2006.02.054. View

16.

Martin J, Colson T, Langlois V, Metcalfe C . Biomarkers of exposure to nanosilver and silver accumulation in yellow perch (Perca flavescens). Environ Toxicol Chem. 2016; 36(5):1211-1220. DOI: 10.1002/etc.3644. View

17.

Farombi E, Adelowo O, Ajimoko Y . Biomarkers of oxidative stress and heavy metal levels as indicators of environmental pollution in African cat fish (Clarias gariepinus) from Nigeria Ogun River. Int J Environ Res Public Health. 2007; 4(2):158-65. PMC: 3728582. DOI: 10.3390/ijerph2007040011. View

18.

Klimaczewski C, Ecker A, Piccoli B, Aschner M, Barbosa N, Rocha J . Peumus boldus attenuates copper-induced toxicity in Drosophila melanogaster. Biomed Pharmacother. 2017; 97:1-8. DOI: 10.1016/j.biopha.2017.09.130. View

19.

Botte E, Jerry D, Codi King S, Smith-Keune C, Negri A . Effects of chlorpyrifos on cholinesterase activity and stress markers in the tropical reef fish Acanthochromis polyacanthus. Mar Pollut Bull. 2011; 65(4-9):384-93. DOI: 10.1016/j.marpolbul.2011.08.020. View

20.

Sandahl J, Jenkins J . Pacific steelhead (Oncorhynchus mykiss) exposed to chlorpyrifos: benchmark concentration estimates for acetylcholinesterase inhibition. Environ Toxicol Chem. 2002; 21(11):2452-8. View