A Novel HPLC Method for Direct Detection of Nitric Oxide Scavengers from Complex Plant Matrices and Its Application to Leaves

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2018 Jul 1

PMID 29958472

Citations 10

Authors

Didier Fraisse

Alexandra Degerine-Roussel

Alexis Bred

Samba Fama Ndoye

Magali Vivier

Catherine Felgines

Francois Senejoux

Affiliations

Soon will be listed here.

Abstract

The present study aimed at developing an original pre-column HPLC assay allowing rapid characterization of nitric oxide (NO) scavengers from complex plant extracts. Sodium nitroprusside (SNP) was employed as a NO donor and spiked with an aqueous extract from leaves prior to HPLC analysis. Relying on the ability of radical scavenging constituents to be oxidized upon reaction with radicals, this assay successfully allowed direct identification of three potential NO scavengers, including verbascoside, isoverbascoside, and luteolin-7--diglucuronide. These three phenolics were also individually assessed for their NO scavenging activities by using a Griess colorimetric assay. With respective IC values of 56 ± 4, 51 ± 3, and 69 ± 5 µg/mL, verbascoside, isoverbascoside, and luteolin-7--diglucuronide were all reported as potent NO scavenging compounds, confirming the efficiency of the SNP spiking HPLC assay. The present method can, thus, be considered as a valuable and effective approach for speeding up the discovery of NO scavenging constituents.

Citing Articles

Antioxidant and Antiproliferative Activities of Phenolic Extracts of (Thunb.) Lindl. Fruits and Leaves.

Pawlowska A, Zurek N, Kapusta I, De Leo M, Braca A Plants (Basel). 2023; 12(18).

PMID: 37765385 PMC: 10536983. DOI: 10.3390/plants12183221.

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Chemical Profiling, Bioactive Properties, and Anticancer and Antimicrobial Potential of L. Leaves.

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Phytochemical Screening and Bioactive Properties of L. Pollen.

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Use of Edible, Medicinal, and Aromatic Plants in Various Health Disorders: A Cross-Sectional Evaluation among Inhabitants in the Area of Thrace, North-Eastern Greece.

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