Genome Sequence of Staphylococcus Aureus Ex1, Isolated from a Patient with Spinal Osteomyelitis

Overview

Journal Genome Announc

Publisher American Society for Microbiology

Date 2018 Jun 30

PMID 29954915

Citations 1

Authors

Helen Morcrette

Marina S Morgan

Audrey Farbos

Paul ONeill

Karen Moore

Richard W Titball

David J Studholme

Affiliations

Soon will be listed here.

Abstract

Here, we present the genome sequence of Ex1, isolated in 2015 from a patient with spinal osteomyelitis at the Royal Devon and Exeter Hospital in the United Kingdom. The availability of the Ex1 genome sequence provides a resource for studying the basis for spinal infection and horizontal gene transfer in .

Citing Articles

Diagnosis of vertebral osteomyelitis.

Maamari J, Tande A, Diehn F, Tai D, Berbari E J Bone Jt Infect. 2022; 7(1):23-32.

PMID: 35136714 PMC: 8814828. DOI: 10.5194/jbji-7-23-2022.

References

Roach D, Burton J, Lee C, Stackhouse B, Butler-Wu S, Cookson B . A Year of Infection in the Intensive Care Unit: Prospective Whole Genome Sequencing of Bacterial Clinical Isolates Reveals Cryptic Transmissions and Novel Microbiota. PLoS Genet. 2015; 11(7):e1005413. PMC: 4521703. DOI: 10.1371/journal.pgen.1005413. View

Phelan J, OSullivan D, Machado D, Ramos J, Whale A, OGrady J . The variability and reproducibility of whole genome sequencing technology for detecting resistance to anti-tuberculous drugs. Genome Med. 2016; 8(1):132. PMC: 5178084. DOI: 10.1186/s13073-016-0385-x. View

Brandenberger M, Tschierske M, Giachino P, Wada A, Berger-Bachi B . Inactivation of a novel three-cistronic operon tcaR-tcaA-tcaB increases teicoplanin resistance in Staphylococcus aureus. Biochim Biophys Acta. 2000; 1523(2-3):135-9. DOI: 10.1016/s0304-4165(00)00133-1. View

Marcais G, Delcher A, Phillippy A, Coston R, Salzberg S, Zimin A . MUMmer4: A fast and versatile genome alignment system. PLoS Comput Biol. 2018; 14(1):e1005944. PMC: 5802927. DOI: 10.1371/journal.pcbi.1005944. View

Grimstrup Joensen K, Scheutz F, Lund O, Hasman H, Kaas R, Nielsen E . Real-time whole-genome sequencing for routine typing, surveillance, and outbreak detection of verotoxigenic Escherichia coli. J Clin Microbiol. 2014; 52(5):1501-10. PMC: 3993690. DOI: 10.1128/JCM.03617-13. View

Rigsby R, Fillgrove K, Beihoffer L, Armstrong R . Fosfomycin resistance proteins: a nexus of glutathione transferases and epoxide hydrolases in a metalloenzyme superfamily. Methods Enzymol. 2006; 401:367-79. DOI: 10.1016/S0076-6879(05)01023-2. View

Garcia-Alcalde F, Okonechnikov K, Carbonell J, Cruz L, Gotz S, Tarazona S . Qualimap: evaluating next-generation sequencing alignment data. Bioinformatics. 2012; 28(20):2678-9. DOI: 10.1093/bioinformatics/bts503. View

Okonechnikov K, Conesa A, Garcia-Alcalde F . Qualimap 2: advanced multi-sample quality control for high-throughput sequencing data. Bioinformatics. 2015; 32(2):292-4. PMC: 4708105. DOI: 10.1093/bioinformatics/btv566. View

Koreen L, Ramaswamy S, Graviss E, Naidich S, Musser J, Kreiswirth B . spa typing method for discriminating among Staphylococcus aureus isolates: implications for use of a single marker to detect genetic micro- and macrovariation. J Clin Microbiol. 2004; 42(2):792-9. PMC: 344479. DOI: 10.1128/JCM.42.2.792-799.2004. View

10.

Li H, Durbin R . Fast and accurate short read alignment with Burrows-Wheeler transform. Bioinformatics. 2009; 25(14):1754-60. PMC: 2705234. DOI: 10.1093/bioinformatics/btp324. View

11.

Angiuoli S, Gussman A, Klimke W, Cochrane G, Field D, Garrity G . Toward an online repository of Standard Operating Procedures (SOPs) for (meta)genomic annotation. OMICS. 2008; 12(2):137-41. PMC: 3196215. DOI: 10.1089/omi.2008.0017. View

12.

Treangen T, Ondov B, Koren S, Phillippy A . The Harvest suite for rapid core-genome alignment and visualization of thousands of intraspecific microbial genomes. Genome Biol. 2014; 15(11):524. PMC: 4262987. DOI: 10.1186/s13059-014-0524-x. View

13.

Bankevich A, Nurk S, Antipov D, Gurevich A, Dvorkin M, Kulikov A . SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol. 2012; 19(5):455-77. PMC: 3342519. DOI: 10.1089/cmb.2012.0021. View