Article: Inhibition of sarcolemmal Na, K, Mg ATPase from the guinea pig heart is not compatible with a homogeneous population of non-interacting ouabain receptors

The inhibition of sarcolemmal Na, K, Mg ATPase from the guinea pig heart by ouabain was evaluated with a coupled enzyme assay. Models of negative cooperativity and of two independent receptors fitted the inhibition data equally well. The analysis was not compatible with a homogeneous population of non-interacting ouabain receptors.

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References

1.

Erdmann E, Schoner W . Ouabain-receptor interactions in (Na+ + K+)-ATPase preparations. II. Effect of cations and nucleotides on rate constants and dissociation constants. Biochim Biophys Acta. 1973; 330(3):302-15. DOI: 10.1016/0005-2736(73)90235-6. View

2.

Jones L, Besch Jr H, Fleming J, McConnaughey M, Watanabe A . Separation of vesicles of cardiac sarcolemma from vesicles of cardiac sarcoplasmic reticulum. Comparative biochemical analysis of component activities. J Biol Chem. 1979; 254(2):530-9. View

3.

Werdan K, Wagenknecht B, Zwissler B, Brown L, Krawietz W, Erdmann E . Cardiac glycoside receptors in cultured heart cells--II. Characterization of a high affinity and a low affinity binding site in heart muscle cells from neonatal rats. Biochem Pharmacol. 1984; 33(12):1873-86. DOI: 10.1016/0006-2952(84)90542-2. View

4.

Taniguchi K, Iida S . Two apparently different ouabain binding sites of (Na + -K + )-ATPase. Biochim Biophys Acta. 1972; 288(1):98-102. DOI: 10.1016/0005-2736(72)90226-x. View

5.

Schwartz A, Allen J, HARIGAYA S . Possible involvement of cardiac Na+, K+-adenosine triphosphatase in the mechanism of action of cardiac glycosides. J Pharmacol Exp Ther. 1969; 168(1):31-41. View

6.

Erdmann E, PHILIPP G, TANNER . Ouabain-receptor interactions in (Na+ + K+)-ATPase preparations. A contribution to the problem of nonlinear Scatchard plots. Biochim Biophys Acta. 1976; 455(2):287-96. DOI: 10.1016/0005-2736(76)90305-9. View

7.

Noel F, GODFRAIND T . Heterogeneity of ouabain specific binding sites and (Na+ + K+)-ATPase inhibition in microsomes from rat heart. Biochem Pharmacol. 1984; 33(1):47-53. DOI: 10.1016/0006-2952(84)90369-1. View

8.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

9.

Onji T, Liu M . Ouabain receptor in isolated adult dog heart myocytes. Arch Biochem Biophys. 1981; 207(1):148-56. DOI: 10.1016/0003-9861(81)90020-5. View

10.

Mansier P, Lelievre L . CA2+-free perfusion of rat heart reveals a (Na+ + K+)ATPase form highly sensitive to ouabain. Nature. 1982; 300(5892):535-7. DOI: 10.1038/300535a0. View

11.

Sweadner K . Two molecular forms of (Na+ + K+)-stimulated ATPase in brain. Separation, and difference in affinity for strophanthidin. J Biol Chem. 1979; 254(13):6060-7. View

12.

Erdmann E, PHILIPP G, Scholz H . Cardiac glycoside receptor, (Na+ + K+)-ATPase activity and force of contraction in rat heart. Biochem Pharmacol. 1980; 29(24):3219-29. DOI: 10.1016/0006-2952(80)90295-6. View

Inhibition of Sarcolemmal Na, K, Mg ATPase from the Guinea Pig Heart is Not Compatible with a Homogeneous Population of Non-interacting Ouabain Receptors