Survival of Immunoglobulins from Human Milk to Preterm Infant Gastric Samples at 1, 2, and 3 H Postprandial

Overview

Journal Neonatology

Publisher Karger

Specialty Gynecology & Obstetrics

Date 2018 Jun 26

PMID 29940583

Citations 11

Authors

Veronique Demers-Mathieu

Mark A Underwood

Robert L Beverly

David C Dallas

Affiliations

Soon will be listed here.

Abstract

Background: Human milk immunoglobulins (Ig) are an important support for the naïve infant immune system; yet the extent to which these proteins survive within the infant digestive tract, particularly for preterm infants, is poorly studied.

Objectives: Our objective was to evaluate the survival of human milk Igs in the preterm stomach across postprandial time.

Methods: Human milk and infant gastric samples were collected from 11 preterm (23-32 weeks gestational age) mother-infant pairs within 7-98 days postnatal age. Preterm gastric samples were collected 1, 2, and 3 h after the beginning of the feeding. Samples were analyzed for concentration of total IgA (secretory IgA [SIgA]/IgA), total secretory component (SC/SIgA/SIgM), total IgM (SIgM/IgM), and IgG via enzyme-linked immunosorbent assay. Ig-chain fragment peptides were determined using peptidomic analysis. One-way analysis of variance with repeated measures followed by Tukey's multiple comparison tests was applied.

Results: Concentrations of total IgA were lower in the gastric contents at 3 h postprandial compared with human milk and gastric contents at 1 and 2 h. Human milk SC/SIgA/SIgM, IgG, and total IgM concentrations remained stable in the preterm stomach across postprandial time. Peptide counts from the Ig alpha-chain and the Ig gamma-chain increased in gastric contents from 1 to 2 h postprandial. Peptide counts from the human milk Ig-chain, Ig-chain, and SC were stable across postprandial time. These peptides from Ig-chains were not present in human milk but were released in the stomach due to their partial degradation.

Conclusions: Human milk total SC (SIgA/SC/SIgM), total IgM, and IgG survived mostly intact through the preterm infant stomach, while total IgA was -partially digested.

Citing Articles

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Anti-SARS-CoV-2 Immunoglobulins in Human Milk after Coronavirus Disease or Vaccination-Time Frame and Duration of Detection in Human Milk and Factors That Affect Their Titers: A Systematic Review.

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Demers-Mathieu V, DaPra C, Mathijssen G, Sela D, Jarvinen K, Seppo A Int J Mol Sci. 2021; 22(4).

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