Molecular Analysis of P Element Behavior in Drosophila Simulans Transformants

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1985 Jan 1

PMID 2993820

Citations 9

Authors

S B Daniels

L D Strausbaugh

R A Armstrong

Affiliations

Soon will be listed here.

Abstract

In this report we describe the successful transformation of Drosophila simulans with an autonomous P element from Drosophila melanogaster without the use of a selectable marker. This result demonstrates that there is no species barrier for P element transposition. Utilizing gel blotting and in situ hybridization techniques, we have monitored the behavior of newly-introduced P elements in several D. simulans transformed lines over twelve generations. In most instances, an overall increase in the number of P elements was observed. An examination of the frequency of P-element-bearing individuals in one line revealed the rapid spread of P elements through the population. Analysis of well-characterized sublines confirmed that P elements increase in number by transposition to new genomic sites. The formation of degenerate elements occurred in at least one case. These observations suggest that P elements may behave similarly in D. melanogaster and D. simulans.

Citing Articles

The element invaded rapidly and caused hybrid dysgenesis in natural populations of in Japan.

Yoshitake Y, Inomata N, Sano M, Kato Y, Itoh M Ecol Evol. 2018; 8(19):9590-9599.

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The recent invasion of natural Drosophila simulans populations by the P-element.

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Rapid spread of transposable p elements in experimental populations of Drosophila melanogaster.

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Germline transformation of Drosophila virilis mediated by the transposable element hobo.

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Characterization of Gandalf, a new inverted-repeat transposable element of Drosophila koepferae.

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