Transposon Donor Plasmids, Based on ColIb-P9, for Use in Pseudomonas Putida and a Variety of Other Gram Negative Bacteria

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1985 Jan 1

PMID 2993815

Citations 11

Authors

G J Boulnois

J M Varley

G S Sharpe

F C Franklin

Affiliations

Soon will be listed here.

Abstract

The properties of pLG221, a derivative of the ColIb plasmid carrying the transposon Tn5 are described. This plasmid can be used to introduce Tn5 by conjugation from Escherichia coli into a variety of Gram negative bacteria outside the host range for maintenance of ColIb. Plasmid pLG221, and a similar plasmid pLG223 carrying Tn10 may be of general utility as vectors for transposon-mediated mutagenesis in a variety of Gram negative bacteria.

Citing Articles

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Conjugative Transfer of Chromosomal Genes between Fluorescent Pseudomonads in the Rhizosphere of Wheat.

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