Protective Effects of Tat-DJ-1 Protein Against Streptozotocin-induced Diabetes in a Mice Model

Overview

Journal BMB Rep

Specialties Biochemistry
Molecular Biology

Date 2018 Jun 26

PMID 29936932

Citations 9

Authors

Hyeon Yeo

Eun Ji Yeo

Min Jea Shin

Yeon Joo Choi

Chi Hern Lee

Hyeok Yil Kwon

Dae Won Kim

Won Sik Eum

Soo Young Choi

Affiliations

Soon will be listed here.

Abstract

A major feature of type 1 diabetes mellitus (T1DM) is hyperglycemia and dysfunction of pancreatic β-cells. In a previous study, we have shown that Tat-DJ-1 protein inhibits pancreatic RINm5F β-cell death caused by oxidative stress. In this study, we examined effects of Tat-DJ-1 protein on streptozotocin (STZ)-induced diabetic mice. Wild type (WT) Tat-DJ-1 protein transduced into pancreas where it markedly inhibited pancreatic β-cell destruction and regulated levels of serum parameters including insulin, alkaline phosphatase (ALP), and free fatty acid (FFA) secretion. In addition, transduced WT Tat-DJ-1 protein significantly inhibited the activation of NF-κB and MAPK (ERK and p38) expression as well as expression of COX-2 and iNOS in STZ exposed pancreas. In contrast, treatment with C106A mutant Tat-DJ-1 protein showed no protective effects. Collectively, our results indicate that WT Tat-DJ-1 protein can significantly ameliorate pancreatic tissues in STZ-induced diabetes in mice. [BMB Reports 2018; 51(7): 362-367].

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