Trans-regulation and Localization of Orthologous Maltose Transporters in the Interspecies Lager Yeast Hybrid

Overview

Journal FEMS Yeast Res

Publisher Oxford University Press

Specialty Microbiology

Date 2018 Jun 23

PMID 29931058

Citations 2

Authors

Virve Vidgren

Brian Gibson

Affiliations

Soon will be listed here.

Abstract

In the interspecies lager yeast hybrid there are MAL loci involved in maltose and maltotriose utilization derived from each parent (Saccharomyces cerevisiae and Saccharomyces eubayanus). We show that trans-regulation across hybrid subgenomes occurs for MAL genes. However, gene expression is less efficient with non-native activators (trans-activation) compared to native activators (cis-activation). MAL genes were induced by maltose and repressed by glucose irrespective of host. Despite the strong expression of S. cerevisiae-type genes in the S. eubayanus host, a very low amount of transporter protein was actually observed in cells. This suggests that proper formation and configuration of the S. cerevisiae transporters is not efficient in S. eubayanus. The S. eubayanus-type Malx1 transporter was present in the plasma membrane in high amounts in all hosts (S. cerevisiae, S. eubayanus and Saccharomyces pastorianus) at all times. However, the S. cerevisiae-type transporters appeared sequentially in the plasma membrane; scMalx1 was localized in the plasma membrane during early to late linear growth and subsequently withdrawn to intracellular compartments. In contrast, the scAgt1 transporter was found in the plasma membrane mainly in the stationary phase of growth. Different localization patterns may explain why certain transporter orthologues in natural S. pastorianus strains were lost to mutation.

Citing Articles

Lager-brewing yeasts in the era of modern genetics.

Gorter de Vries A, Pronk J, Daran J FEMS Yeast Res. 2019; 19(7).

PMID: 31553794 PMC: 6790113. DOI: 10.1093/femsyr/foz063.

Himalayan Genome Sequences Reveal Genetic Markers Explaining Heterotic Maltotriose Consumption by Saccharomyces pastorianus Hybrids.

Brouwers N, Brickwedde A, Gorter de Vries A, van den Broek M, Weening S, van den Eijnden L Appl Environ Microbiol. 2019; 85(22).

PMID: 31519660 PMC: 6821976. DOI: 10.1128/AEM.01516-19.

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