Raman Spectroscopy Detects Changes in Bone Mineral Quality and Collagen Cross-linkage in Staphylococcus Infected Human Bone

Overview

Journal Sci Rep

Specialty Science

Date 2018 Jun 22

PMID 29925892

Citations 46

Authors

Mohamed Khalid

Tanujjal Bora

Ahmed Al Ghaithi

Sharanjit Thukral

Joydeep Dutta

Affiliations

Soon will be listed here.

Abstract

Diagnosis of osteomyelitis presents a formidable challenge. Lack of pathognomonic clinical sign(s) and diagnostic tests that can diagnose osteomyelitis at an early stage contribute to this difficulty. If the diagnosis is not made early, the disease becomes very difficult to eradicate and can lead to limb threatening and potentially life-threatening complications. Staphylococcus aureus is the most common organism causing osteomyelitis. Raman Spectroscopy provides information about molecular vibration that could potentially be harnessed as a spectral signature for cellular changes in specific pathologic conditions. In this study we describe a technique using Raman spectroscopy that could potentially be used to diagnose staphylococcal osteomyelitis. Human bone samples were co-cultured with Staphylococcus aureus (S. aureus) and the effects of bacterial growth on bone quality were then monitored using Raman spectroscopy. A major drop in the bone mineral quality and crystallinity was observed in the infected bones compared to the controls. S. aureus infection was also found to alter the collagen cross-linking. Our study shows that specific spectral signatures are present for the cause as well as the effect of staphylococcal osteomyelitis, opening the possibility of developing a useful diagnostic modality for early and rapid diagnosis of this condition.

Citing Articles

Combination of mesenchymal stem cell sheet with poly-caprolactone nanofibrous mat and Gelfoam increased osteogenesis capacity in rat calvarial defect.

Banimohamad-Shotorbani B, Rahbarghazi R, Jarolmasjed S, Mehdipour A, Shafaei H Bioimpacts. 2025; 15:30006.

PMID: 39963571 PMC: 11830138. DOI: 10.34172/bi.30006.

Qualitative comparison of decalcifiers for mouse bone cryosections for subsequent biophotonic analysis.

Mandal S, Motganhalli Ravikumar R, Tannert A, Urbanek A, Guliev R, Naumann M Sci Rep. 2025; 15(1):1153.

PMID: 39774725 PMC: 11707355. DOI: 10.1038/s41598-024-84330-2.

Harnessing Raman spectroscopy and multimodal imaging of cartilage for osteoarthritis diagnosis.

Crisford A, Cook H, Bourdakos K, Venkateswaran S, Dunlop D, Oreffo R Sci Rep. 2024; 14(1):31466.

PMID: 39733214 PMC: 11682361. DOI: 10.1038/s41598-024-83155-3.

BMP-4 and fetuin A in systemic sclerosis patients with or without calcinosis.

Lofaro F, Giuggioli D, Bonacorsi S, Orlandi M, Spinella A, de Pinto M Front Immunol. 2024; 15():1502324.

PMID: 39697336 PMC: 11653211. DOI: 10.3389/fimmu.2024.1502324.

Lead Toxicity and Maternal Exposure: Characterisation of Alveolar Bone Changes on Offspring Rats.

Rodrigues M, Souza-Monteiro D, Nazario R, Aragao W, Chemelo V, Eiro-Quirino L Biol Trace Elem Res. 2024; .

PMID: 39453595 DOI: 10.1007/s12011-024-04412-0.

References

Gamsjaeger S, Hofstetter B, Zwettler E, Recker R, Gasser J, Eriksen E . Effects of 3 years treatment with once-yearly zoledronic acid on the kinetics of bone matrix maturation in osteoporotic patients. Osteoporos Int. 2012; 24(1):339-47. DOI: 10.1007/s00198-012-2202-8. View

Bandekar J . Amide modes and protein conformation. Biochim Biophys Acta. 1992; 1120(2):123-43. DOI: 10.1016/0167-4838(92)90261-b. View

Esmonde-White K, Esmonde-White F, Holmes C, Morris M, Roessler B . Alterations to bone mineral composition as an early indication of osteomyelitis in the diabetic foot. Diabetes Care. 2013; 36(11):3652-4. PMC: 3816922. DOI: 10.2337/dc13-0510. View

Yerramshetty J, Lind C, Akkus O . The compositional and physicochemical homogeneity of male femoral cortex increases after the sixth decade. Bone. 2006; 39(6):1236-43. DOI: 10.1016/j.bone.2006.06.002. View

Beattie J, Cummins N, Caraher C, ODriscoll O, Bansal A, Eastell R . Raman Spectroscopic Analysis of Fingernail Clippings Can Help Differentiate Between Postmenopausal Women Who Have and Have Not Suffered a Fracture. Clin Med Insights Arthritis Musculoskelet Disord. 2016; 9:109-16. PMC: 4939848. DOI: 10.4137/CMAMD.S38493. View

. Bone mineral crystal size. Osteoporos Int. 2003; 14 Suppl 5:S16-20. DOI: 10.1007/s00198-003-1468-2. View

Wright J, Nair S . Interaction of staphylococci with bone. Int J Med Microbiol. 2009; 300(2-3):193-204. PMC: 2814006. DOI: 10.1016/j.ijmm.2009.10.003. View

Gasior-Glogowska M, Komorowska M, Hanuza J, Ptak M, Kobielarz M . Structural alteration of collagen fibres--spectroscopic and mechanical studies. Acta Bioeng Biomech. 2011; 12(4):55-62. View

Sinha B, Francois P, Nusse O, Foti M, Hartford O, Vaudaux P . Fibronectin-binding protein acts as Staphylococcus aureus invasin via fibronectin bridging to integrin alpha5beta1. Cell Microbiol. 2001; 1(2):101-17. DOI: 10.1046/j.1462-5822.1999.00011.x. View

10.

Iping Petterson I, Day J, Fullwood L, Gardner B, Stone N . Characterisation of a fibre optic Raman probe within a hypodermic needle. Anal Bioanal Chem. 2015; 407(27):8311-20. DOI: 10.1007/s00216-015-9021-7. View

11.

Busse B, Bale H, Zimmermann E, Panganiban B, Barth H, Carriero A . Vitamin D deficiency induces early signs of aging in human bone, increasing the risk of fracture. Sci Transl Med. 2013; 5(193):193ra88. DOI: 10.1126/scitranslmed.3006286. View

12.

Scholtes-Timmerman M, Willemse-Erix H, Schut T, van Belkum A, Puppels G, Maquelin K . A novel approach to correct variations in Raman spectra due to photo-bleachable cellular components. Analyst. 2009; 134(2):387-93. DOI: 10.1039/b811596f. View

13.

Kim G, Cole J, Boskey A, Baker S, van der Meulen M . Reduced tissue-level stiffness and mineralization in osteoporotic cancellous bone. Calcif Tissue Int. 2014; 95(2):125-31. PMC: 4104238. DOI: 10.1007/s00223-014-9873-4. View

14.

Berger E, Saunders N, Wang L, Friedman J . Sickle cell disease in children: differentiating osteomyelitis from vaso-occlusive crisis. Arch Pediatr Adolesc Med. 2009; 163(3):251-5. DOI: 10.1001/archpediatrics.2008.545. View

15.

McConnell D . The Problem of the Carbonate Apatites. Science. 1954; 119(3104):913-4. DOI: 10.1126/science.119.3104.913-a. View

16.

Maiti N, Apetri M, Zagorski M, Carey P, Anderson V . Raman spectroscopic characterization of secondary structure in natively unfolded proteins: alpha-synuclein. J Am Chem Soc. 2004; 126(8):2399-408. DOI: 10.1021/ja0356176. View

17.

Kong K, Kendall C, Stone N, Notingher I . Raman spectroscopy for medical diagnostics--From in-vitro biofluid assays to in-vivo cancer detection. Adv Drug Deliv Rev. 2015; 89:121-34. DOI: 10.1016/j.addr.2015.03.009. View

18.

Freeman J, Wopenka B, Silva M, Pasteris J . Raman spectroscopic detection of changes in bioapatite in mouse femora as a function of age and in vitro fluoride treatment. Calcif Tissue Int. 2001; 68(3):156-62. DOI: 10.1007/s002230001206. View

19.

Morris M, Mandair G . Raman assessment of bone quality. Clin Orthop Relat Res. 2010; 469(8):2160-9. PMC: 3126952. DOI: 10.1007/s11999-010-1692-y. View

20.

McCreadie B, Morris M, Chen T, Rao D, Finney W, Widjaja E . Bone tissue compositional differences in women with and without osteoporotic fracture. Bone. 2006; 39(6):1190-5. DOI: 10.1016/j.bone.2006.06.008. View