Integrated Assessment of Differentially Expressed Plasma MicroRNAs in Subtypes of Nonsyndromic Orofacial Clefts

Overview

Journal Medicine (Baltimore)

Specialty General Medicine

Date 2018 Jun 21

PMID 29924053

Citations 4

Authors

Ni Wu

Jun Yan

Tao Han

Jijun Zou

Weimin Shen

Affiliations

Soon will be listed here.

Abstract

Background: Orofacial clefts include cleft lip only (CLO), cleft palate only (CPO), and cleft lip with palate (CLP). Previously, we reported the expression profile of plasma microRNAs in CLO, CPO, and CLP, respectively. However, the interaction of each subtype remains poorly investigated.

Methods: In this study, we integrated the expression profiles of plasma miRNAs in these 3 subtypes, and assessed the distinct and overlapping dysregulated miRNAs using Venn diagrams. Their respective target genes reported in the literature were further analyzed using pathway analysis.

Results And Conclusion: The results showed that distinct or overlapping signaling pathways were involved in CLO, CPO, and CLP. The common key gene targets reflected functional relationships to the Wnt, Notch, TGF-beta, and Hedgehog signaling pathways. Further studies should examine the mechanism of the potential target genes, which may provide new avenues for future clinical prevention and therapy.

Citing Articles

Phenytoin Inhibits Cell Proliferation through microRNA-196a-5p in Mouse Lip Mesenchymal Cells.

Yoshioka H, Ramakrishnan S, Suzuki A, Iwata J Int J Mol Sci. 2021; 22(4).

PMID: 33572377 PMC: 7916186. DOI: 10.3390/ijms22041746.

Extracellular Matrix Composition and Remodeling: Current Perspectives on Secondary Palate Formation, Cleft Lip/Palate, and Palatal Reconstruction.

Paiva K, Maas C, Dos Santos P, Granjeiro J, Letra A Front Cell Dev Biol. 2020; 7:340.

PMID: 31921852 PMC: 6923686. DOI: 10.3389/fcell.2019.00340.

MicroRNAs as Epigenetic Targets of Cigarette Smoke During Embryonic Development.

Seelan R, Greene R, Pisano M Microrna. 2019; 9(3):168-173.

PMID: 31556862 PMC: 7365999. DOI: 10.2174/2211536608666190926114704.

Upregulated miR‑203a‑3p and its potential molecular mechanism in breast cancer: A study based on bioinformatics analyses and a comprehensive meta‑analysis.

Cai K, Feng C, Zhao J, He R, Ma J, Zhong J Mol Med Rep. 2018; 18(6):4994-5008.

PMID: 30320391 PMC: 6236224. DOI: 10.3892/mmr.2018.9543.

References

Deiuliis J . MicroRNAs as regulators of metabolic disease: pathophysiologic significance and emerging role as biomarkers and therapeutics. Int J Obes (Lond). 2015; 40(1):88-101. PMC: 4722234. DOI: 10.1038/ijo.2015.170. View

Jugessur A, Shi M, Gjessing H, Lie R, Wilcox A, Weinberg C . Fetal genetic risk of isolated cleft lip only versus isolated cleft lip and palate: a subphenotype analysis using two population-based studies of orofacial clefts in Scandinavia. Birth Defects Res A Clin Mol Teratol. 2011; 91(2):85-92. PMC: 3407039. DOI: 10.1002/bdra.20747. View

Leslie E, Marazita M . Genetics of cleft lip and cleft palate. Am J Med Genet C Semin Med Genet. 2013; 163C(4):246-58. PMC: 3925974. DOI: 10.1002/ajmg.c.31381. View

Carlson J, Standley J, Petrin A, Shaffer J, Butali A, Buxo C . Identification of 16q21 as a modifier of nonsyndromic orofacial cleft phenotypes. Genet Epidemiol. 2017; 41(8):887-897. PMC: 5728176. DOI: 10.1002/gepi.22090. View

Li J, Zou J, Li Q, Chen L, Gao Y, Yan H . Assessment of differentially expressed plasma microRNAs in nonsyndromic cleft palate and nonsyndromic cleft lip with cleft palate. Oncotarget. 2016; 7(52):86266-86279. PMC: 5349912. DOI: 10.18632/oncotarget.13379. View

Wang J, Bai Y, Li H, Greene S, Klysik E, Yu W . MicroRNA-17-92, a direct Ap-2α transcriptional target, modulates T-box factor activity in orofacial clefting. PLoS Genet. 2013; 9(9):e1003785. PMC: 3777996. DOI: 10.1371/journal.pgen.1003785. View

Peng H, Chang N, Chen K, Lu J, Chang P, Chang S . Nonsynonymous variants in MYH9 and ABCA4 are the most frequent risk loci associated with nonsyndromic orofacial cleft in Taiwanese population. BMC Med Genet. 2016; 17(1):59. PMC: 4986225. DOI: 10.1186/s12881-016-0322-2. View

Pelikan R, Iwata J, Suzuki A, Chai Y, Hacia J . Identification of candidate downstream targets of TGFβ signaling during palate development by genome-wide transcript profiling. J Cell Biochem. 2012; 114(4):796-807. PMC: 3777336. DOI: 10.1002/jcb.24417. View

Ladd-Acosta C, Beaty T . Integrating RNA Expression Identifies Candidate Gene for Orofacial Clefts. J Dent Res. 2017; 97(1):31-32. DOI: 10.1177/0022034517735806. View

10.

Sheehy N, Cordes K, White M, Ivey K, Srivastava D . The neural crest-enriched microRNA miR-452 regulates epithelial-mesenchymal signaling in the first pharyngeal arch. Development. 2010; 137(24):4307-16. PMC: 2990216. DOI: 10.1242/dev.052647. View

11.

Rahimov F, Jugessur A, Murray J . Genetics of nonsyndromic orofacial clefts. Cleft Palate Craniofac J. 2011; 49(1):73-91. PMC: 3437188. DOI: 10.1597/10-178. View

12.

Dixon M, Marazita M, Beaty T, Murray J . Cleft lip and palate: understanding genetic and environmental influences. Nat Rev Genet. 2011; 12(3):167-78. PMC: 3086810. DOI: 10.1038/nrg2933. View

13.

Wang M, Yuan Y, Wang Z, Liu D, Wang Z, Sun F . Prevalence of Orofacial Clefts among Live Births in China: A Systematic Review and Meta-Analysis. Birth Defects Res. 2017; 109(13):1011-1019. PMC: 5944334. DOI: 10.1002/bdr2.1043. View

14.

Milger K, Gotschke J, Krause L, Nathan P, Alessandrini F, Tufman A . Identification of a plasma miRNA biomarker signature for allergic asthma: A translational approach. Allergy. 2017; 72(12):1962-1971. DOI: 10.1111/all.13205. View

15.

Kondo S, Schutte B, Richardson R, Bjork B, Knight A, Watanabe Y . Mutations in IRF6 cause Van der Woude and popliteal pterygium syndromes. Nat Genet. 2002; 32(2):285-9. PMC: 3169431. DOI: 10.1038/ng985. View

16.

Metzis V, Courtney A, Kerr M, Ferguson C, Galeano M, Parton R . Patched1 is required in neural crest cells for the prevention of orofacial clefts. Hum Mol Genet. 2013; 22(24):5026-35. DOI: 10.1093/hmg/ddt353. View

17.

Schwarzenbach H, Nishida N, Calin G, Pantel K . Clinical relevance of circulating cell-free microRNAs in cancer. Nat Rev Clin Oncol. 2014; 11(3):145-56. DOI: 10.1038/nrclinonc.2014.5. View

18.

Yu Y, Zuo X, He M, Gao J, Fu Y, Qin C . Genome-wide analyses of non-syndromic cleft lip with palate identify 14 novel loci and genetic heterogeneity. Nat Commun. 2017; 8:14364. PMC: 5333091. DOI: 10.1038/ncomms14364. View

19.

Dougherty M, Kamel G, Grimaldi M, Gfrerer L, Shubinets V, Ethier R . Distinct requirements for wnt9a and irf6 in extension and integration mechanisms during zebrafish palate morphogenesis. Development. 2012; 140(1):76-81. PMC: 6514306. DOI: 10.1242/dev.080473. View

20.

Meng L, Bian Z, Torensma R, von den Hoff J . Biological mechanisms in palatogenesis and cleft palate. J Dent Res. 2009; 88(1):22-33. DOI: 10.1177/0022034508327868. View