Induced Pluripotent Stem Cell-derived Mesenchymal Stem Cells Activate Quiescent T Cells and Elevate Regulatory T Cell Response Via NF-κB in Allergic Rhinitis Patients

Overview

Journal Stem Cell Res Ther

Publisher Biomed Central

Date 2018 Jun 21

PMID 29921316

Citations 28

Authors

Xing-Liang Fan

Qing-Xiang Zeng

Xin Li

Cheng-Lin Li

Zhi-Bin Xu

Xue-Quan Deng

Jianbo Shi

Dong Chen

Song Guo Zheng

Qing-Ling Fu

Affiliations

Soon will be listed here.

Abstract

Background: It has been demonstrated previously that induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells (MSCs) have immunosuppressive effects on activated T cells. However, the effects of iPSC-MSCs on quiescent T cells are still unknown. The aim of this study was to identify the immunomodulatory role of iPSC-MSCs on resting peripheral blood mononuclear cells (PBMCs) from allergic rhinitis (AR) patients.

Methods: PBMCs were cocultured with iPSC-MSCs without any stimulation, following which lymphocyte proliferation, activation of T cells, T1/T2 and regulatory T (Treg) cell differentiation, and Treg cell function were analyzed. The roles of soluble factors and cell-cell contact were examined to investigate the mechanisms involved.

Results: iPSC-MSCs promoted the proliferation of resting lymphocytes, activated CD4 and CD8 T cells, and upregulated and activated Treg cells without any additional stimulation. In addition, iPSC-MSCs balanced biased T1/T2 cytokine levels. Cell-cell contact was confirmed to be a possible mechanism involved. NF-κB was identified to play an important role in the immunomodulatory effects of iPSC-MSCs on quiescent T cells.

Conclusions: iPSC-MSCs activate quiescent T cells and elevate regulatory T-cell response in AR patients, suggesting different immunomodulatory functions of iPSC-MSCs according to the phases of diseases. Therefore, iPSC-MSCs are a potential therapeutic candidate for treating allergic airway inflammation.

Citing Articles

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