Potential Role of the Src Gene Product in Inhibition of Gap-junctional Communication in NIH/3T3 Cells

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1985 Aug 1

PMID 2991919

Citations 17

Authors

C C Chang

J E Trosko

H J Kung

D Bombick

F Matsumura

Affiliations

Soon will be listed here.

Abstract

The effects of the src gene on the activity of protein kinase C and intercellular communication have been studied in transformed NIH/3T3 clones isolated from soft agar following transfection with the plasmid carrying the v-src gene (psrc-11). Six transformed clones that were studied contained newly incorporated v-src genes in the genome, had an increased amount of pp60src, and showed enhanced activities of protein kinase C. Intercellular communication, studied by observing with autoradiography the transfer of [3H]uridine nucleotide from prelabeled donor cells to recipient cells in contact, was found to be reduced in transformed clones as compared to parental NIH/3T3 cells. Treatment with phorbol 12-myristate 13-acetate was also found to increase protein kinase C activity and to reduce intercellular communication in normal NIH/3T3 cells. These results suggest that the v-src gene product, in a manner similar to some of the powerful tumor promoters, may directly or indirectly affect cell-cell communication.

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