Isolation and Characterization of Novel Broad Host Range Bacteriophages of O1 from Bengal

Overview

Journal J Glob Infect Dis

Date 2018 Jun 19

PMID 29910569

Citations 4

Authors

Sounak Sarkar

Mayukh Das

Tushar Suvra Bhowmick

Hemanta Koley

Robert Atterbury

Alok K Chakrabarti

Banwarilal L Sarkar

Affiliations

Soon will be listed here.

Abstract

Objectives: We have isolated a total of five newer cholera phages which are novel broad host range to incorporate with the existing phage typing schemes for an extended typing scheme.

Materials And Methods: These newly isolated phages were well characterized including the electron micrograph. A total of 300 strains were isolated from the different endemic region in India were included in phage typing study.

Results: These phages were found different from the existing phages. Electron microscopic results showed that the phages belonged to myophage and podophage group. Characterization of the phages based on pH, temperature, and organic solvent sensitivity showed differences among the phages used in this study. All the strains of O1 were typeable (100%) with the five set of cholera phages. Of these, 40% strains were clustered under Type-1.

Conclusion: The newer phages are novel and broad host range and will be useful to incorporate with the existing phage typing system for more precisely discriminate the strains of Vibrio cholerae.

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