Quantitative Trait Locus Mapping and Candidate Gene Analysis for Wilt Resistance Using Chromosomal Segment Introgressed Line

Overview

Journal Front Plant Sci

Date 2018 Jun 15

PMID 29899750

Citations 9

Authors

Jun Zhao

Jianguang Liu

Jianwen Xu

Liang Zhao

Qiaojuan Wu

Songhua Xiao

Affiliations

Soon will be listed here.

Abstract

wilt (VW) is a soil-borne fungal disease that is caused by Kleb and seriously damages cotton production annually in China. To date, many efforts have been made to improve the resistance of upland cotton against VW, but little progress has been achieved because of a lack of resistant upland cotton to VW. is known to carry high resistance to VW; however, it is difficult to transfer the resistance trait from to upland cotton because of linkage drag and distortion in the interspecific hybrid. In this study, a chromosomal segment introgression line (CSIL), SuVR043, containing a single and homozygous chromosome segment of cv. H7124 D04 (Chr 22), was created and used to construct an F population for mapping of VW resistance quantitative trait loci (QTLs) in the greenhouse. Two major resistance QTLs against nondefoliating isolate Bp2, called qVW-Bp2-1 and qVW-Bp2-2, which were flanked by the markers cgr6409-ZHX37 and ZHX57-ZHX70 and explained an average of 16.38 and 22.36% of the observed phenotypic variation, respectively, were detected in three independent replicate experiments. The genetic distances from cgr6409 to ZHX37 and from ZHX57 to ZHX70 were 2.4 and 0.8 cM, respectively. By analyzing the genome sequence of the qVW-Bp2-1 and qVW-Bp2-2 regions, we determined that the accurate physical distances from cgr6409 to ZHX37 and from ZHX57 to ZHX70 in the genome are 254 and 140 kb, and that those spans 36 and 20 putative genes, respectively. The results of the expression analysis showed significant differences in the expression profiles of , and among cv. H7124, CSIL SuVR043 and acc. Sumian 8 at different times after inoculation with isolate Bp2. Virus-induced gene silencing (VIGS) analysis showed that silencing of and decreased H7124 and CSIL SuVR043 resistance to VW. These results form a solid foundation for fine mapping and cloning of resistance genes in the substituted segment and will provide valuable assistance in future efforts to breed for VW resistance.

Citing Articles

A recessive LRR-RLK gene causes hybrid breakdown in cotton.

Xu P, Xu J, Guo Q, Xu Z, Ji W, Yu H Theor Appl Genet. 2023; 136(9):189.

PMID: 37582982 DOI: 10.1007/s00122-023-04427-6.

Genome-wide association analysis reveals a novel pathway mediated by a dual-TIR domain protein for pathogen resistance in cotton.

Zhang Y, Zhang Y, Ge X, Yuan Y, Jin Y, Wang Y Genome Biol. 2023; 24(1):111.

PMID: 37165460 PMC: 10170703. DOI: 10.1186/s13059-023-02950-9.

Heat Shock Transcription Factor GhHSFB2a Is Crucial for Cotton Resistance to .

Liu L, Wang Q, Zhu L, Guo H, Cheng H, Su X Int J Mol Sci. 2023; 24(3).

PMID: 36768168 PMC: 9916287. DOI: 10.3390/ijms24031845.

Membrane Localized GbTMEM214s Participate in Modulating Cotton Resistance to Verticillium Wilt.

Zhao J, Xu J, Wang Y, Liu J, Dong C, Zhao L Plants (Basel). 2022; 11(18).

PMID: 36145743 PMC: 9505811. DOI: 10.3390/plants11182342.

QTL mapping and candidate gene prediction for fiber yield and quality traits in a high-generation cotton chromosome substitution line with Gossypium barbadense segments.

Lu Q, Li P, Yang R, Xiao X, Li Z, Wu Q Mol Genet Genomics. 2022; 297(2):287-301.

PMID: 35187584 DOI: 10.1007/s00438-021-01833-7.

References

Lees J, Messa M, Sun E, Wheeler H, Torta F, Wenk M . Lipid transport by TMEM24 at ER-plasma membrane contacts regulates pulsatile insulin secretion. Science. 2017; 355(6326). PMC: 5414417. DOI: 10.1126/science.aah6171. View

Gao X, Li F, Li M, Kianinejad A, Dever J, Wheeler T . Cotton GhBAK1 mediates Verticillium wilt resistance and cell death. J Integr Plant Biol. 2013; 55(7):586-96. PMC: 4395461. DOI: 10.1111/jipb.12064. View

Li W, Shao M, Yang J, Zhong W, Okada K, Yamane H . Oscyp71Z2 involves diterpenoid phytoalexin biosynthesis that contributes to bacterial blight resistance in rice. Plant Sci. 2013; 207:98-107. DOI: 10.1016/j.plantsci.2013.02.005. View

Kawchuk L, Hachey J, Lynch D, Kulcsar F, van Rooijen G, Waterer D . Tomato Ve disease resistance genes encode cell surface-like receptors. Proc Natl Acad Sci U S A. 2001; 98(11):6511-5. PMC: 33499. DOI: 10.1073/pnas.091114198. View

de Jonge R, van Esse H, Maruthachalam K, Bolton M, Santhanam P, Saber M . Tomato immune receptor Ve1 recognizes effector of multiple fungal pathogens uncovered by genome and RNA sequencing. Proc Natl Acad Sci U S A. 2012; 109(13):5110-5. PMC: 3323992. DOI: 10.1073/pnas.1119623109. View

Zhang Y, Wang X, Li Y, Wu L, Zhou H, Zhang G . Ectopic expression of a novel Ser/Thr protein kinase from cotton (Gossypium barbadense), enhances resistance to Verticillium dahliae infection and oxidative stress in Arabidopsis. Plant Cell Rep. 2013; 32(11):1703-13. DOI: 10.1007/s00299-013-1481-7. View

Katoh M, Katoh M . Characterization of human TMEM16G gene in silico. Int J Mol Med. 2004; 14(4):759-64. View

Jun Z, Zhang Z, Gao Y, Zhou L, Fang L, Chen X . Overexpression of GbRLK, a putative receptor-like kinase gene, improved cotton tolerance to Verticillium wilt. Sci Rep. 2015; 5:15048. PMC: 4597213. DOI: 10.1038/srep15048. View

Chai Q, Shang X, Wu S, Zhu G, Cheng C, Cai C . 5-Aminolevulinic Acid Dehydratase Gene Dosage Affects Programmed Cell Death and Immunity. Plant Physiol. 2017; 175(1):511-528. PMC: 5580774. DOI: 10.1104/pp.17.00816. View

10.

Johansson A, Staal J, Dixelius C . Early responses in the Arabidopsis-Verticillium longisporum pathosystem are dependent on NDR1, JA- and ET-associated signals via cytosolic NPR1 and RFO1. Mol Plant Microbe Interact. 2006; 19(9):958-69. DOI: 10.1094/MPMI-19-0958. View

11.

Xu L, Zhang W, He X, Liu M, Zhang K, Shaban M . Functional characterization of cotton genes responsive to Verticillium dahliae through bioinformatics and reverse genetics strategies. J Exp Bot. 2014; 65(22):6679-92. PMC: 4246195. DOI: 10.1093/jxb/eru393. View

12.

Zamir D . Improving plant breeding with exotic genetic libraries. Nat Rev Genet. 2001; 2(12):983-9. DOI: 10.1038/35103590. View

13.

Guo W, Jin L, Miao Y, He X, Hu Q, Guo K . An ethylene response-related factor, GbERF1-like, from Gossypium barbadense improves resistance to Verticillium dahliae via activating lignin synthesis. Plant Mol Biol. 2016; 91(3):305-18. DOI: 10.1007/s11103-016-0467-6. View

14.

Zhang Z, Zhao J, Ding L, Zou L, Li Y, Chen G . Constitutive expression of a novel antimicrobial protein, Hcm1, confers resistance to both Verticillium and Fusarium wilts in cotton. Sci Rep. 2016; 6:20773. PMC: 4746735. DOI: 10.1038/srep20773. View

15.

Smigocki A, Wilson D . Pest and disease resistance enhanced by heterologous suppression of a Nicotiana plumbaginifolia cytochrome P450 gene CYP72A2. Biotechnol Lett. 2005; 26(23):1809-14. DOI: 10.1007/s10529-004-4615-8. View

16.

Mo H, Wang X, Zhang Y, Zhang G, Zhang J, Ma Z . Cotton polyamine oxidase is required for spermine and camalexin signalling in the defence response to Verticillium dahliae. Plant J. 2015; 83(6):962-75. DOI: 10.1111/tpj.12941. View

17.

Gao X, Wheeler T, Li Z, Kenerley C, He P, Shan L . Silencing GhNDR1 and GhMKK2 compromises cotton resistance to Verticillium wilt. Plant J. 2011; 66(2):293-305. PMC: 3078967. DOI: 10.1111/j.1365-313X.2011.04491.x. View

18.

Conesa A, Gotz S, Garcia-Gomez J, Terol J, Talon M, Robles M . Blast2GO: a universal tool for annotation, visualization and analysis in functional genomics research. Bioinformatics. 2005; 21(18):3674-6. DOI: 10.1093/bioinformatics/bti610. View

19.

Li Y, Han L, Wang H, Zhang J, Sun S, Feng D . The Thioredoxin GbNRX1 Plays a Crucial Role in Homeostasis of Apoplastic Reactive Oxygen Species in Response to Verticillium dahliae Infection in Cotton. Plant Physiol. 2016; 170(4):2392-406. PMC: 4825149. DOI: 10.1104/pp.15.01930. View

20.

Zhang T, Hu Y, Jiang W, Fang L, Guan X, Chen J . Sequencing of allotetraploid cotton (Gossypium hirsutum L. acc. TM-1) provides a resource for fiber improvement. Nat Biotechnol. 2015; 33(5):531-7. DOI: 10.1038/nbt.3207. View