Totally Synthetic Microperoxidase-11

Overview

Journal R Soc Open Sci

Specialty Science

Date 2018 Jun 13

PMID 29892416

Citations 2

Authors

Junichi Tanabe

Koji Nakano

Ryutaro Hirata

Toshiki Himeno

Ryoichi Ishimatsu

Toshihiko Imato

Hirotaka Okabe

Naoki Matsuda

Affiliations

Soon will be listed here.

Abstract

A totally synthetic microperoxidase-11 (MP-11) is reported. Accordingly, the undecapeptide (VQKCAQCHTVE) was synthesized by solid-phase peptide synthesis followed by the thiol-ene click reaction with haemin for reconstitution. High-speed atomic force microscopy measurement conducted in water confirmed the protein reconstitution by visualizing the morphological differences as animated molecular images. The synthetic MP-11 showed a considerable magnitude of catalytic activity (27%) against the natural MP-11 in the oxidation of 3,3',5,5'-tetramethylbenzidine by hydrogen peroxide, whereas it showed very low (2.7%) activity of a synthetic variant with a point mutation (VQKCAQC TVE, H8M). Slab waveguide spectroscopic measurements revealed that the ferrous/ferric redox reaction occurred by the direct electron transfer with specific spectral changes. Indeed, if hydrogen peroxide existed in the solution phase, the peroxidase-modified electrode showed catalytic current-voltage behaviour regardless of whether it was prepared using natural MP-11 or the synthetic MP-11. If a substrate recycling reaction was assumed, computer simulation well reproduced the experimental curves to give a global set of electrocatalytic reaction parameters. In any of the experiments, the synthetic MP-11 and natural MP-11 gave almost identical results. Our approach will be a convenient means of preparing MP-11, as well as its mutants, that does not rely on nature.

Citing Articles

Electrochemical Biosensors Employing Natural and Artificial Heme Peroxidases on Semiconductors.

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PMID: 32630267 PMC: 7374321. DOI: 10.3390/s20133692.

A Novel Peroxidase Mimics and Ameliorates Alzheimer's Disease-Related Pathology and Cognitive Decline in Mice.

Xu J, Wang K, Yuan Y, Li H, Zhang R, Guan S Int J Mol Sci. 2018; 19(11).

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