Notochordal Cell Matrix As a Bioactive Lubricant for the Osteoarthritic Joint

Overview

Journal Sci Rep

Specialty Science

Date 2018 Jun 13

PMID 29891965

Citations 5

Authors

S A H de Vries

M van Doeselaar

H J Kaper

P K Sharma

K Ito

Affiliations

Soon will be listed here.

Abstract

Notochordal cell derived matrix (NCM) can induce regenerative effects on nucleus pulposus cells and may exert such effects on chondrocytes as well. Furthermore, when dissolved at low concentrations, NCM forms a viscous fluid with potential lubricating properties. Therefore, this study tests the feasibility of the use of NCM as a regenerative lubricant for the osteoarthritic joint. Chondrocyte-seeded alginate beads were cultured in base medium (BM), BM with NCM (NCM), or BM with TGF-β1 (TGF), as well as BM and NCM treated with IL-1β. NCM increased GAG deposition and cell proliferation (stronger than TGF), and GAG/DNA ratio and hydroxyproline content (similar to TGF). These effects were maintained in the presence of IL-1β. Moreover, NCM mitigated expression of IL-1β-induced IL-6, IL-8, ADAMTS-5 and MMP-13. Reciprocating sliding friction tests of cartilage on glass were performed to test NCM's lubricating properties relative to hyaluronic acid (HA), and showed a dose-dependent reduction in coefficient of friction with NCM, similar to HA. NCM has anabolic and anti-inflammatory effects on chondrocytes, as well as lubricating properties. Therefore, intra-articular NCM injection may have potential as a treatment to minimize pain while restoring the affected cartilage tissue in the osteoarthritic joint.

Citing Articles

Notochordal cells: A potential therapeutic option for intervertebral disc degeneration.

Li Y, Zhang H, Zhu D, Yang F, Wang Z, Wei Z Cell Prolif. 2023; 57(2):e13541.

PMID: 37697480 PMC: 10849793. DOI: 10.1111/cpr.13541.

Detergent-Free Decellularization of Notochordal Cell-Derived Matrix Yields a Regenerative, Injectable, and Swellable Biomaterial.

Schmitz T, van Doeselaar M, Tryfonidou M, Ito K ACS Biomater Sci Eng. 2022; 8(9):3912-3923.

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Notochordal Cell-Based Treatment Strategies and Their Potential in Intervertebral Disc Regeneration.

Bach F, Poramba-Liyanage D, Riemers F, Guicheux J, Camus A, Iatridis J Front Cell Dev Biol. 2022; 9:780749.

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Fluorescence microscopy visualization of the roughness-induced transition between lubrication regimes.

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