Diazinon Interrupts Ovarian Steroidogenic Acute Regulatory () Gene Transcription in Gonadotropin-Stimulated Rat Model

Overview

Journal Iran J Pharm Res

Publisher Brieflands

Specialty Pharmacology

Date 2018 Jun 9

PMID 29881411

Citations 2

Authors

Asma Siavashpour

Younes Ghasemi

Bahman Khalvati

Fereshteh Jeivad

Negar Azarpira

Hossein Niknahad

Affiliations

Soon will be listed here.

Abstract

Organophosphate pesticides are considered as endocrine disruptors that interfere with reproductive functions. The (CL) is a transient endocrine gland that produces progesterone, a crucial hormone for a successful beginning and maintenance of pregnancy Steroidogenic acute regulatory protein () facilitates the rate-limiting transfer of cholesterol from the outer mitochondrial membrane to the inner organelle membranes. We investigated the effect of Diazinon (DZN), an organophosphate, on mRNA expression by Sybergreen Real Time-PCR in a time-dependent manner in luteal phase. Fifty immature female Wistar rats (24-day-old) were injected with a single injection of Pregnant mare's Serum Gonadotropin (PMSG) followed by a single injection of human Chorionic Gonadotropin (hCG), 48 h later. Then, DZN was administered in a single dose (70 mg/kg bw, I.P), controls received only the vehicle, 12 h post-hCG injection. Ovaries were collected in 4 h. intervals from 8 to 24 h post-hCG injection. Then, hCG stimulation transcript levels of gene were significantly altered in the hormone-stimulated rats following DZN treatment. In addition, histological study showed that the CL diameter in DZN-treated group was smaller than control group ( = 0.000). Our findings suggest that the critical step in the function of CL is disrupted by DZN and may correlates with female reproductive damage.

Citing Articles

Effects of diazinon on the ovarian tissue of rats: a histochemical and ultrastructural study.

Abou Hasan F, Mutlu H, Ozdemir I, Kotil T J Mol Histol. 2024; 55(6):1211-1223.

PMID: 39283561 DOI: 10.1007/s10735-024-10261-x.

Updates on molecular and environmental determinants of luteal progesterone production.

DeWitt N, Whirledge S, Kallen A Mol Cell Endocrinol. 2020; 515:110930.

PMID: 32610113 PMC: 7484338. DOI: 10.1016/j.mce.2020.110930.

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