Accelerated Neural Differentiation of Mouse Embryonic Stem Cells on Aligned GYIGSR-functionalized Nanofibers

Overview

Journal Acta Biomater

Publisher Elsevier

Specialty Biomedical Engineering

Date 2018 Jun 8

PMID 29879551

Citations 18

Authors

Elena A Silantyeva

Wafaa Nasir

Jacqueline Carpenter

Olivia Manahan

Matthew L Becker

Rebecca K Willits

Affiliations

Soon will be listed here.

Abstract

Statement Of Significance: In this paper, we report the use of GYIGSR-functionalized poly(ε-caprolactone) aligned nanofibers as a tool to accelerate the neural lineage commitment and differentiation of D3 mouse embryonic stem cells. The results indicate that functional nanofiber substrates promote faster differentiation than laminin coated substrates. The data suggest that aligned nanofibers and post-electrospinning surface modification with bioactive species can be combined to produce translationally relevant xeno-free substrates for stem cell therapy. Future development efforts are focused on additional bioactive species that are able to function as surrogates for other xenogenic factors found in differentiation media.

Citing Articles

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References

Yamada Y, Kleinman H . Functional domains of cell adhesion molecules. Curr Opin Cell Biol. 1992; 4(5):819-23. DOI: 10.1016/0955-0674(92)90105-l. View

Corey J, Lin D, Mycek K, Chen Q, Samuel S, Feldman E . Aligned electrospun nanofibers specify the direction of dorsal root ganglia neurite growth. J Biomed Mater Res A. 2007; 83(3):636-45. DOI: 10.1002/jbm.a.31285. View

Smith Callahan L, Xie S, Barker I, Zheng J, Reneker D, Dove A . Directed differentiation and neurite extension of mouse embryonic stem cell on aligned poly(lactide) nanofibers functionalized with YIGSR peptide. Biomaterials. 2013; 34(36):9089-95. DOI: 10.1016/j.biomaterials.2013.08.028. View

Kleinman H, Cannon F, Laurie G, Hassell J, Aumailley M, Terranova V . Biological activities of laminin. J Cell Biochem. 1985; 27(4):317-25. DOI: 10.1002/jcb.240270402. View

Beatty K, Fisk J, Smart B, Lu Y, Szychowski J, Hangauer M . Live-cell imaging of cellular proteins by a strain-promoted azide-alkyne cycloaddition. Chembiochem. 2010; 11(15):2092-5. PMC: 3069858. DOI: 10.1002/cbic.201000419. View

Manchineella S, Thrivikraman G, Basu B, Govindaraju T . Surface-Functionalized Silk Fibroin Films as a Platform To Guide Neuron-like Differentiation of Human Mesenchymal Stem Cells. ACS Appl Mater Interfaces. 2016; 8(35):22849-59. DOI: 10.1021/acsami.6b06403. View

Haque A, Yue X, Motazedian A, Tagawa Y, Akaike T . Characterization and neural differentiation of mouse embryonic and induced pluripotent stem cells on cadherin-based substrata. Biomaterials. 2012; 33(20):5094-106. DOI: 10.1016/j.biomaterials.2012.04.003. View

Liu L, Yuan Q, Shi J, Li X, Jung D, Wang L . Chemically-defined scaffolds created with electrospun synthetic nanofibers to maintain mouse embryonic stem cell culture under feeder-free conditions. Biotechnol Lett. 2012; 34(10):1951-7. DOI: 10.1007/s10529-012-0973-9. View

Wongpaiboonwattana W, Stavridis M . Neural differentiation of mouse embryonic stem cells in serum-free monolayer culture. J Vis Exp. 2015; (99):e52823. PMC: 4542683. DOI: 10.3791/52823. View

10.

Grandjean C, Boutonnier A, Guerreiro C, Fournier J, Mulard L . On the preparation of carbohydrate-protein conjugates using the traceless Staudinger ligation. J Org Chem. 2005; 70(18):7123-32. DOI: 10.1021/jo0505472. View

11.

Sperling L, Reis K, Pozzobon L, Girardi C, Pranke P . Influence of random and oriented electrospun fibrous poly(lactic-co-glycolic acid) scaffolds on neural differentiation of mouse embryonic stem cells. J Biomed Mater Res A. 2017; 105(5):1333-1345. DOI: 10.1002/jbm.a.36012. View

12.

Patel S, Kurpinski K, Quigley R, Gao H, Hsiao B, Poo M . Bioactive nanofibers: synergistic effects of nanotopography and chemical signaling on cell guidance. Nano Lett. 2007; 7(7):2122-8. DOI: 10.1021/nl071182z. View

13.

Ingavle G, Leach J . Advancements in electrospinning of polymeric nanofibrous scaffolds for tissue engineering. Tissue Eng Part B Rev. 2013; 20(4):277-93. DOI: 10.1089/ten.TEB.2013.0276. View

14.

Xie J, MacEwan M, Schwartz A, Xia Y . Electrospun nanofibers for neural tissue engineering. Nanoscale. 2010; 2(1):35-44. DOI: 10.1039/b9nr00243j. View

15.

Zheng J, Kontoveros D, Lin F, Hua G, Reneker D, Becker M . Enhanced Schwann cell attachment and alignment using one-pot "dual click" GRGDS and YIGSR derivatized nanofibers. Biomacromolecules. 2014; 16(1):357-63. PMC: 5953569. DOI: 10.1021/bm501552t. View

16.

Mullen P, Ritchie A, Langdon S, Miller W . Effect of Matrigel on the tumorigenicity of human breast and ovarian carcinoma cell lines. Int J Cancer. 1996; 67(6):816-20. DOI: 10.1002/(SICI)1097-0215(19960917)67:6<816::AID-IJC10>3.0.CO;2-#. View

17.

Sharifi F, Patel B, Dzuilko A, Montazami R, Sakaguchi D, Hashemi N . Polycaprolactone Microfibrous Scaffolds to Navigate Neural Stem Cells. Biomacromolecules. 2016; 17(10):3287-3297. DOI: 10.1021/acs.biomac.6b01028. View

18.

Teo W, He W, Ramakrishna S . Electrospun scaffold tailored for tissue-specific extracellular matrix. Biotechnol J. 2006; 1(9):918-29. DOI: 10.1002/biot.200600044. View

19.

Aumailley M, Smyth N . The role of laminins in basement membrane function. J Anat. 1998; 193 ( Pt 1):1-21. PMC: 1467819. DOI: 10.1046/j.1469-7580.1998.19310001.x. View

20.

Ghasemi-Mobarakeh L, Prabhakaran M, Morshed M, Nasr-Esfahani M, Ramakrishna S . Electrospun poly(epsilon-caprolactone)/gelatin nanofibrous scaffolds for nerve tissue engineering. Biomaterials. 2008; 29(34):4532-9. DOI: 10.1016/j.biomaterials.2008.08.007. View