Processing of Donor DNA During Haemophilus Influenzae Transformation: Analysis Using a Model Plasmid System

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1985 Jun 1

PMID 2987941

Citations 18

Authors

M L Pifer

H O Smith

Affiliations

Soon will be listed here.

Abstract

A plasmid system was used to investigate the processing of donor DNA during transformation of competent Haemophilus influenzae. Using biochemical and genetic methods, we have determined that portion of a donor plasmid molecule that, on average, can become integrated into a homologous recipient plasmid during transformation. Our results show that (i) transformation efficiency decreases linearly with donor DNA length over the range of 11 to 3.5 kilobase pairs, (ii) transformation efficiency decreases exponentially with size for donor molecules less than 3.5 kilobase pairs in length, and (iii) 5'-end label, but not 3'-end label, can be specifically incorporated into the resident homologous region. We present a model for donor processing during entry that encompasses and explains these observations.

Citing Articles

gene expression profile of during human pneumonia.

Polland L, Ryden H, Su Y, Paulsson M Microbiol Spectr. 2023; :e0163923.

PMID: 37707456 PMC: 10581191. DOI: 10.1128/spectrum.01639-23.

Genome-wide analysis of DNA uptake across the outer membrane of naturally competent .

Mora M, Mell J, Ehrlich G, Ehrlich R, Redfield R iScience. 2021; 24(1):102007.

PMID: 33490915 PMC: 7811141. DOI: 10.1016/j.isci.2020.102007.

Native Plasmid-Encoded Mercury Resistance Genes Are Functional and Demonstrate Natural Transformation in Environmental Bacterial Isolates.

Kothari A, Soneja D, Tang A, Carlson H, Deutschbauer A, Mukhopadhyay A mSystems. 2019; 4(6).

PMID: 31848306 PMC: 6918032. DOI: 10.1128/mSystems.00588-19.

Evolution of Bacterial Gene Transfer Agents.

Redfield R, Soucy S Front Microbiol. 2018; 9:2527.

PMID: 30410473 PMC: 6209664. DOI: 10.3389/fmicb.2018.02527.

Mechanisms and Regulation of Extracellular DNA Release and Its Biological Roles in Microbial Communities.

Ibanez de Aldecoa A, Zafra O, Gonzalez-Pastor J Front Microbiol. 2017; 8:1390.

PMID: 28798731 PMC: 5527159. DOI: 10.3389/fmicb.2017.01390.

References

Repine J, Pfenninger O, TALMAGE D, Berger E, PETTIJOHN D . Dimethyl sulfoxide prevents DNA nicking mediated by ionizing radiation or iron/hydrogen peroxide-generated hydroxyl radical. Proc Natl Acad Sci U S A. 1981; 78(2):1001-3. PMC: 319933. DOI: 10.1073/pnas.78.2.1001. View

HERRIOTT R, Meyer E, Vogt M . Defined nongrowth media for stage II development of competence in Haemophilus influenzae. J Bacteriol. 1970; 101(2):517-24. PMC: 284936. DOI: 10.1128/jb.101.2.517-524.1970. View

Danner D, Deich R, Sisco K, Smith H . An eleven-base-pair sequence determines the specificity of DNA uptake in Haemophilus transformation. Gene. 1980; 11(3-4):311-8. DOI: 10.1016/0378-1119(80)90071-2. View

Lataste H, Claverys J, Sicard A . Relation between the transforming activity of a marker and its proximity to the end of the DNA particle. Mol Gen Genet. 1981; 183(1):199-201. DOI: 10.1007/BF00270163. View

Hirt B . Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967; 26(2):365-9. DOI: 10.1016/0022-2836(67)90307-5. View

Morrison D, Guild W . Transformation and deoxyribonucleic acid size: extent of degradation on entry varies with size of donor. J Bacteriol. 1972; 112(3):1157-68. PMC: 251544. DOI: 10.1128/jb.112.3.1157-1168.1972. View

Kahn M, BARANY F, Smith H . Transformasomes: specialized membranous structures that protect DNA during Haemophilus transformation. Proc Natl Acad Sci U S A. 1983; 80(22):6927-31. PMC: 390099. DOI: 10.1073/pnas.80.22.6927. View

Cato Jr A, Guild W . Transformation and DNA size. I. Activity of fragments of defined size and a fit to a random double cross-over model. J Mol Biol. 1968; 37(1):157-78. DOI: 10.1016/0022-2836(68)90080-6. View

Smith H, Danner D, Deich R . Genetic transformation. Annu Rev Biochem. 1981; 50:41-68. DOI: 10.1146/annurev.bi.50.070181.000353. View

10.

Litt M, MARMUR J, Ephrussi-Taylor H, Doty P . THE DEPENDENCE OF PNEUMOCOCCAL TRANSFORMATION ON THE MOLECULAR WEIGHT OF DEOXYRIBOSE NUCLEIC ACID. Proc Natl Acad Sci U S A. 1958; 44(2):144-52. PMC: 335379. DOI: 10.1073/pnas.44.2.144. View

11.

GOODGAL S . DNA uptake in Haemophilus transformation. Annu Rev Genet. 1982; 16:169-92. DOI: 10.1146/annurev.ge.16.120182.001125. View

12.

Bertrand K, Postle K, Wray Jr L, Reznikoff W . Overlapping divergent promoters control expression of Tn10 tetracycline resistance. Gene. 1983; 23(2):149-56. DOI: 10.1016/0378-1119(83)90046-x. View

13.

Harris-Warrick R, Elkana Y, Ehrlich S, LEDERBERG J . Electrophoretic separation of Bacillus subtilis genes. Proc Natl Acad Sci U S A. 1975; 72(6):2207-11. PMC: 432726. DOI: 10.1073/pnas.72.6.2207. View

14.

Kahn M, Smith H . Transformation in Haemophilus: a problem in membrane biology. J Membr Biol. 1984; 81(2):89-103. DOI: 10.1007/BF01868974. View

15.

Danner D, Pifer M . Plasmid cloning vectors resistant to ampicillin and tetracycline which can replicate in both E. coli and Haemophilus cells. Gene. 1982; 18(1):101-5. DOI: 10.1016/0378-1119(82)90062-2. View

16.

Beattie K, Wakil A, Driggers P . Action of restriction endonucleases on transforming DNA of Haemophilus influenzae. J Bacteriol. 1982; 152(1):332-7. PMC: 221409. DOI: 10.1128/jb.152.1.332-337.1982. View

17.

Notani N, GOODGAL S . On the nature of recombinants formed during transformation in Hemophilus influenzae. J Gen Physiol. 1966; 49(6):197-209. PMC: 2195535. DOI: 10.1085/jgp.49.6.197. View

18.

Birnboim H, DOLY J . A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979; 7(6):1513-23. PMC: 342324. DOI: 10.1093/nar/7.6.1513. View

19.

STUY J . Fate of transforming DNA in the Haemophilus influenzae transformation system. J Mol Biol. 1965; 13(2):554-70. DOI: 10.1016/s0022-2836(65)80117-6. View

20.

Lefevre J, Claverys J, Sicard A . Donor deoxyribonucleic acid length and marker effect in pneumococcal transformation. J Bacteriol. 1979; 138(1):80-6. PMC: 218240. DOI: 10.1128/jb.138.1.80-86.1979. View