BE-PLUS: a New Base Editing Tool with Broadened Editing Window and Enhanced Fidelity

Overview

Journal Cell Res

Specialty Cell Biology

Date 2018 Jun 8

PMID 29875396

Citations 62

Authors

Wen Jiang

Songjie Feng

Shisheng Huang

Wenxia Yu

Guanglei Li

Guang Yang

Yajing Liu

Yu Zhang

Lei Zhang

Yu Hou

Jia Chen

Jieping Chen

Xingxu Huang

Affiliations

Soon will be listed here.

Abstract

Base editor (BE), containing a cytidine deaminase and catalytically defective Cas9, has been widely used to perform base editing. However, the narrow editing window of BE limits its utility. Here, we developed a new editing technology named as base editor for programming larger C to U (T) scope (BE-PLUS) by fusing 10 copies of GCN4 peptide to nCas9(D10A) for recruiting scFv-APOBEC-UGI-GB1 to the target sites. The new system achieves base editing with a broadened window, resulting in an increased genome-targeting scope. Interestingly, the new system yielded much fewer unwanted indels and non-C-to-T conversions. We also demonstrated its potential use in gene disruption across the whole genome through induction of stop codons (iSTOP). Taken together, the BE-PLUS system offers a new editing tool with increased editing window and enhanced fidelity.

Citing Articles

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