Growing Oocyte-specific Transcription-dependent De Novo DNA Methylation at the Imprinted Zrsr1-DMR

Overview

Journal Epigenetics Chromatin

Publisher Biomed Central

Specialties Biochemistry
Genetics

Date 2018 Jun 8

PMID 29875017

Citations 10

Authors

Keiichiro Joh

Fumikazu Matsuhisa

Shuji Kitajima

Kenichi Nishioka

Ken Higashimoto

Hitomi Yatsuki

Tomohiro Kono

Haruhiko Koseki

Hidenobu Soejima

Affiliations

Soon will be listed here.

Abstract

Background: Zrsr1 is a paternally expressed imprinted gene located in the first intron of Commd1, and the Zrsr1 promoter resides in a differentially methylated region (DMR) that is maternally methylated in the oocyte. However, a mechanism for the establishment of the methylation has remained obscure. Commd1 is transcribed in the opposite direction to Zrsr1 with predominant maternal expression, especially in the adult brain.

Results: We found Commed1 transcribed through the DMR in the growing oocyte. Zrsr1-DMR methylation was abolished by the prevention of Commd1 transcription. Furthermore, methylation did not occur at the artificially unmethylated maternal Zrsr1-DMR during embryonic development when transcription through the DMR was restored in the zygote. Loss of methylation at the maternal Zrsr1-DMR resulted in biallelic Zrsr1 expression and reduced the extent of the predominant maternal expression of Commd1.

Conclusions: These results indicate that the establishment of methylation at Zrsr1-DMR occurs in a transcription-dependent and oocyte-specific manner and caused Zrsr1 imprinting by repressing maternal expression. The predominant maternal expression of Commd1 is likely caused by transcriptional interference by paternal Zrsr1 expression.

Citing Articles

Identification of responsible sequences which mutations cause maternal H19-ICR hypermethylation with Beckwith-Wiedemann syndrome-like overgrowth.

Hara S, Matsuhisa F, Kitajima S, Yatsuki H, Kubiura-Ichimaru M, Higashimoto K Commun Biol. 2024; 7(1):1605.

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Role of transcription in imprint establishment in the male and female germ lines.

Liao J, Szabo P Epigenomics. 2023; 16(2):127-136.

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Establishment of paternal methylation imprint at the imprinting control region.

Liao J, Song S, Gusscott S, Fu Z, VanderKolk I, Busscher B Sci Adv. 2023; 9(36):eadi2050.

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Conservation and divergence of canonical and non-canonical imprinting in murids.

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Tissue-specific Grb10/Ddc insulator drives allelic architecture for cardiac development.

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