Diminished Circulating Plasma and Elevated Lymph Node Culture Supernatant Levels of IL-10 Family Cytokines in Tuberculous Lymphadenitis

Overview

Journal Cytokine

Specialties Allergy & Immunology
Biology

Date 2018 Jun 7

PMID 29871780

Citations 2

Authors

Gokul Raj Kathamuthu

Nathella Pavan Kumar

Kadar Moideen

Dhanaraj Baskaran

Syed Hissar

Basavegowdanadoddi M Shrinivasa

Rathinam Sridhar

Subash Babu

Affiliations

Soon will be listed here.

Abstract

Background: IL-10 family cytokines are associated with the host immune response to pulmonary tuberculosis (PTB), but their association with host response in tuberculous lymphadenitis (TBL) is not known.

Methods: Hence, we examined the circulating levels of the whole panel of IL-10 family cytokines in TBL (n = 44) and compared them to the levels in PTB (n = 44) and healthy control (HC, n = 44) individuals. We also assessed the pre and post-treatment cytokine levels in TBL individuals following the completion of anti-tuberculosis treatment (ATT). Next, we also compared the levels of IL-10 family cytokine in circulation versus lymph node (LN) culture supernatants in a subset of TBL individuals (n = 22). Finally, we also measured the levels of IL-10 family cytokines in tuberculosis antigen (purified protein derivative, PPD) stimulated and unstimulated LN culture supernatants.

Results: TBL individuals exhibit significantly decreased levels of IL-10, IL-19, IL-20, IL-24, IL-28B and IL-29 in the circulation when compared to PTB (except IL-10) and HC (except IL-20 and IL-28B) and significantly increased levels of IL-22 when compared to PTB individuals. Following ATT, TBL individuals exhibit significantly elevated levels of IL-10, IL-19, IL-20, IL-24, IL-28B and IL-29 and significantly diminished levels of IL-26. Similarly, TBL individuals also exhibited significantly increased levels of IL-10, IL-19, IL-20, IL-24, IL-28A and IL-29 in LN culture supernatants compared to plasma and significantly decreased levels of IL-22. This was associated with enhanced levels of IL-19, IL-20, IL-24, IL-28B and IL-29 upon PPD stimulation of LN cultures.

Conclusions: Therefore, we demonstrate that TBL is associated with significantly diminished plasma and elevated LN culture supernatant levels of most of the IL-10 family cytokines. This to our knowledge is the first comprehensive examination of IL-10 family cytokines in TBL.

Citing Articles

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PMID: 37215496 PMC: 10199325. DOI: 10.1515/biol-2022-0594.

Interleukin-26 activates macrophages and facilitates killing of Mycobacterium tuberculosis.

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