Synergy of Sodium Nitroprusside and Nitrate in Inhibiting the Activity of Sulfate Reducing Bacteria in Oil-Containing Bioreactors

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2018 Jun 6

PMID 29867883

Citations 4

Authors

Tekle T Fida

Johanna Voordouw

Maryam Ataeian

Manuel Kleiner

Gloria Okpala

Jaspreet Mand

Gerrit Voordouw

Affiliations

Soon will be listed here.

Abstract

Sodium nitroprusside (SNP) disrupts microbial biofilms through the release of nitric oxide (NO). The actions of SNP on bacteria have been mostly limited to the genera , , and . There are no reports of its biocidal action on sulfate-reducing bacteria (SRB), which couple the reduction of sulfate to sulfide with the oxidation of organic electron donors. Here, we report the inhibition and kill of SRB by low SNP concentrations [0.05 mM (15 ppm)] depending on biomass concentration. Chemical reaction of SNP with sulfide did not compromise its efficacy. SNP was more effective than five biocides commonly used to control SRB. Souring, the SRB activity in oil reservoirs, is often controlled by injection of nitrate. Control of SRB-mediated souring in oil-containing bioreactors was inhibited by 4 mM (340 ppm) of sodium nitrate, but required only 0.05 mM (15 ppm) of SNP. Interestingly, nitrate and SNP were found to be highly synergistic with 0.003 mM (1 ppm) of SNP and 1 mM (85 ppm) of sodium nitrate being sufficient in inhibiting souring. Hence, using SNP as an additive may greatly increase the efficacy of nitrate injection in oil reservoirs.

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References

Kjellerup B, Veeh R, Sumithraratne P, Thomsen T, Buckingham-Meyer K, Frolund B . Monitoring of microbial souring in chemically treated, produced-water biofilm systems using molecular techniques. J Ind Microbiol Biotechnol. 2005; 32(4):163-70. DOI: 10.1007/s10295-005-0222-5. View

Bertrand R, Danielson D, Gong V, Olynik B, Eze M . Sodium nitroprusside may modulate Escherichia coli antioxidant enzyme expression by interacting with the ferric uptake regulator. Med Hypotheses. 2011; 78(1):130-3. DOI: 10.1016/j.mehy.2011.10.007. View

Okpala G, Chen C, Fida T, Voordouw G . Effect of Thermophilic Nitrate Reduction on Sulfide Production in High Temperature Oil Reservoir Samples. Front Microbiol. 2017; 8:1573. PMC: 5581841. DOI: 10.3389/fmicb.2017.01573. View

Gardner L, Stewart P . Action of glutaraldehyde and nitrite against sulfate-reducing bacterial biofilms. J Ind Microbiol Biotechnol. 2002; 29(6):354-60. DOI: 10.1038/sj.jim.7000284. View

Moore C, Nakano M, Wang T, Ye R, Helmann J . Response of Bacillus subtilis to nitric oxide and the nitrosating agent sodium nitroprusside. J Bacteriol. 2004; 186(14):4655-64. PMC: 438601. DOI: 10.1128/JB.186.14.4655-4664.2004. View

Fida T, Breugelmans P, Lavigne R, Coronado E, Johnson D, van der Meer J . Exposure to solute stress affects genome-wide expression but not the polycyclic aromatic hydrocarbon-degrading activity of Sphingomonas sp. strain LH128 in biofilms. Appl Environ Microbiol. 2012; 78(23):8311-20. PMC: 3497376. DOI: 10.1128/AEM.02516-12. View

Srinivasan S, Gillies A, Chang L, Thompson A, Gestwicki J . Molecular chaperones DnaK and DnaJ share predicted binding sites on most proteins in the E. coli proteome. Mol Biosyst. 2012; 8(9):2323-33. PMC: 3462289. DOI: 10.1039/c2mb25145k. View

Quiroga S, Almaraz A, Amorebieta V, Perissinotti L, Olabe J . Addition and redox reactivity of hydrogen sulfides (H2S/HS⁻) with nitroprusside: new chemistry of nitrososulfide ligands. Chemistry. 2011; 17(15):4145-56. DOI: 10.1002/chem.201002322. View

Voordouw G, Grigoryan A, Lambo A, Lin S, Park H, Jack T . Sulfide remediation by pulsed injection of nitrate into a low temperature Canadian heavy oil reservoir. Environ Sci Technol. 2009; 43(24):9512-8. DOI: 10.1021/es902211j. View

10.

Koslyk J, Ducci R, Novak E, Zetola V, Lange M . Sodium nitroprusside: low price and safe drug to control BP during thrombolysis in AIS. Arq Neuropsiquiatr. 2015; 73(9):755-8. DOI: 10.1590/0004-282X20150104. View

11.

Haveman S, Greene E, Stilwell C, Voordouw J, Voordouw G . Physiological and gene expression analysis of inhibition of Desulfovibrio vulgaris hildenborough by nitrite. J Bacteriol. 2004; 186(23):7944-50. PMC: 529081. DOI: 10.1128/JB.186.23.7944-7950.2004. View

12.

Carlson H, Kuehl J, Hazra A, Justice N, Stoeva M, Sczesnak A . Mechanisms of direct inhibition of the respiratory sulfate-reduction pathway by (per)chlorate and nitrate. ISME J. 2014; 9(6):1295-305. PMC: 4438318. DOI: 10.1038/ismej.2014.216. View

13.

Barnes R, Bandi R, Wong W, Barraud N, McDougald D, Fane A . Optimal dosing regimen of nitric oxide donor compounds for the reduction of Pseudomonas aeruginosa biofilm and isolates from wastewater membranes. Biofouling. 2013; 29(2):203-12. DOI: 10.1080/08927014.2012.760069. View

14.

Rabus R, Venceslau S, Wohlbrand L, Voordouw G, Wall J, Pereira I . A Post-Genomic View of the Ecophysiology, Catabolism and Biotechnological Relevance of Sulphate-Reducing Prokaryotes. Adv Microb Physiol. 2015; 66:55-321. DOI: 10.1016/bs.ampbs.2015.05.002. View

15.

Le Gall J, Payne W, Chen L, Liu M, Xavier A . Localization and specificity of cytochromes and other electron transfer proteins from sulfate-reducing bacteria. Biochimie. 1994; 76(7):655-65. DOI: 10.1016/0300-9084(94)90142-2. View

16.

Widdel F, Pfennig N . Studies on dissimilatory sulfate-reducing bacteria that decompose fatty acids. I. Isolation of new sulfate-reducing bacteria enriched with acetate from saline environments. Description of Desulfobacter postgatei gen. nov., sp. nov. Arch Microbiol. 1981; 129(5):395-400. DOI: 10.1007/BF00406470. View

17.

Xue Y, Voordouw G . Control of Microbial Sulfide Production with Biocides and Nitrate in Oil Reservoir Simulating Bioreactors. Front Microbiol. 2015; 6:1387. PMC: 4672050. DOI: 10.3389/fmicb.2015.01387. View

18.

Bodtker G, Thorstenson T, Lillebo B, Thorbjornsen B, Ulvoen R, Sunde E . The effect of long-term nitrate treatment on SRB activity, corrosion rate and bacterial community composition in offshore water injection systems. J Ind Microbiol Biotechnol. 2008; 35(12):1625-36. DOI: 10.1007/s10295-008-0406-x. View

19.

Fida T, Gassara F, Voordouw G . Biodegradation of isopropanol and acetone under denitrifying conditions by Thauera sp. TK001 for nitrate-mediated microbially enhanced oil recovery. J Hazard Mater. 2017; 334:68-75. DOI: 10.1016/j.jhazmat.2017.03.061. View

20.

TRUEPER H, Schlegel H . SULPHUR METABOLISM IN THIORHODACEAE. I. QUANTITATIVE MEASUREMENTS ON GROWING CELLS OF CHROMATIUM OKENII. Antonie Van Leeuwenhoek. 1964; 30:225-38. DOI: 10.1007/BF02046728. View