A Novel Method to Image Macropinocytosis

Overview

Journal Front Neurosci

Date 2018 Jun 6

PMID 29867333

Citations 6

Authors

Lunhao Chen

Daxiao Cheng

Jiachen Chu

Ting Zhang

Zhuoer Dong

Huifang Lou

Liya Zhu

Yijun Liu

Affiliations

Soon will be listed here.

Abstract

Here we described an experimental protocol for imaging of macropinocytosis and subsequent intracellular events. By microinjection, we delivered fluorescence dextrans together with or without ATPγS into transparent melanogaster embryos. Using a confocal microscope for live imaging, we monitored the generation of dextran-positive macropinosomes and subsequent intracellular events. Our protocol provides a continent and reliable way for investigating macropinocytosis and its underlying mechanisms, especially when combined with genetic strategies.

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