A Large-tumor-antigen-specific Monoclonal Antibody Inhibits DNA Replication of Simian Virus 40 Minichromosomes in an in Vitro Elongation System

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1985 May 1

PMID 2985809

Citations 30

Authors

H Stahl

P Droge

H Zentgraf

R Knippers

Affiliations

Soon will be listed here.

Abstract

In productively infected cells, a fraction of large-tumor antigen (T antigen) is tightly bound to replicating simian virus 40 (SV40) minichromosomes and does not dissociate at salt concentrations of greater than 1 M NaCl. We present electronmicrograms demonstrating the presence of T antigen on the replicated sections of replicating SV40 minichromosomes. We also show that the fraction of tightly bound T antigen is recognized by antibodies from mouse tumor serum and, more specifically, by a particular T-antigen-specific monoclonal antibody, PAb 1630. A second T-antigen-specific monoclonal antibody, PAb 101, does not react with the T-antigen fraction remaining on replicating SV40 chromatin at high salt concentrations. We used an in vitro replication system which allows, via semiconservative DNA replication, the completion of in vivo-initiated replicative intermediate DNA molecules. We show that monoclonal antibody PAb 1630, but not monoclonal antibody PAb 101, inhibits viral DNA replication. We discuss the possibility that SV40 T antigen may play a role in chain elongation during SV40 chromatin replication.

Citing Articles

Activation of CREB/ATF sites by polyomavirus large T antigen.

Love T, De Jesus R, Kean J, Sheng Q, Leger A, Schaffhausen B J Virol. 2005; 79(7):4180-90.

PMID: 15767419 PMC: 1061560. DOI: 10.1128/JVI.79.7.4180-4190.2005.

Topoisomerase activity is associated with purified SV40 T antigen.

Mann K Nucleic Acids Res. 1993; 21(8):1697-704.

PMID: 8388094 PMC: 309403. DOI: 10.1093/nar/21.8.1697.

Protein domains connect cell cycle stimulation directly to initiation of DNA replication.

Gjorup O, Rose P, Holman P, Bockus B, Schaffhausen B Proc Natl Acad Sci U S A. 1994; 91(25):12125-9.

PMID: 7991595 PMC: 45389. DOI: 10.1073/pnas.91.25.12125.

Unwinding of chromatin by the SV40 large T antigen DNA helicase.

Ramsperger U, Stahl H EMBO J. 1995; 14(13):3215-25.

PMID: 7621834 PMC: 394383. DOI: 10.1002/j.1460-2075.1995.tb07324.x.

Application of an immunoprecipitation procedure to the study of SV40 tumor antigen interaction with mouse genomic DNA sequences.

Pollwein P, Wagner S, Knippers R Nucleic Acids Res. 1987; 15(23):9741-59.

PMID: 3697081 PMC: 306528. DOI: 10.1093/nar/15.23.9741.

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