CircFGFR4 Promotes Differentiation of Myoblasts Via Binding MiR-107 to Relieve Its Inhibition of Wnt3a

Overview

Journal Mol Ther Nucleic Acids

Publisher Cell Press

Date 2018 Jun 3

PMID 29858062

Citations 87

Authors

Hui Li

Xuefeng Wei

Jiameng Yang

Dong Dong

Dan Hao

Yongzhen Huang

Xianyong Lan

Martin Plath

Chuzhao Lei

Yun Ma

Fengpeng Lin

Yueyu Bai

Hong Chen

Affiliations

Soon will be listed here.

Abstract

Muscle development is regulated under a series of complicate processes, and non-coding RNAs, such as microRNAs (miRNAs) and circular RNAs (circRNAs), have been reported to play important roles in regulating myoblast proliferation and differentiation. We found that miR-107 expression was high in skeletal muscle of Qinchuan cattle. Overexpression of miR-107 inhibited bovine myoblasts differentiation and protected cells from apoptosis. Wnt3a was identified as a target of miR-107 by luciferase activity, real-time qPCR, and western blotting assays. Knockdown of Wnt3a inhibited bovine myoblasts differentiation and apoptosis, and this effect was similar to miR-107 overexpression. We also found circFGFR4 to promote myoblasts differentiation and to induce cell apoptosis. Via luciferase screening and RNA pull-down assays, circFGFR4 was observed to sponge miR-107. Overexpression of circFGFR4 increased the expression of Wnt3a, whereas this effect was abolished by miR-107. These results demonstrated that circFGFR4 binding miR-107 promotes cell differentiation via targeting Wnt3a in bovine primary myoblasts.

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