A DNA Barcode Library for Ground Beetles of Germany: the Genus Bonelli, 1810 (Insecta, Coleoptera, Carabidae)

Overview

Journal Zookeys

Date 2018 Jun 2

PMID 29853775

Citations 7

Authors

Michael J Raupach

Karsten Hannig

Jerome Moriniere

Lars Hendrich

Affiliations

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Abstract

The genus Bonelli, 1810 is a very speciose and taxonomically difficult genus of the Carabidae. The identification of many of the species is accomplished with considerable difficulty, in particular for females and immature stages. In this study the effectiveness of DNA barcoding, the most popular method for molecular species identification, was examined to discriminate various species of this genus from Central Europe. DNA barcodes from 690 individuals and 47 species were analysed, including sequences from previous studies and more than 350 newly generated DNA barcodes. Our analysis revealed unique BINs for 38 species (81%). Interspecific K2P distances below 2.2% were found for three species pairs and one species trio, including haplotype sharing between / and /. This study represents another step in generating an extensive reference library of DNA barcodes for carabids, highly valuable bioindicators for characterizing disturbances in various habitats.

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References

Kimura M . A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol. 1980; 16(2):111-20. DOI: 10.1007/BF01731581. View

Schmidt S, Schmid-Egger C, Moriniere J, Haszprunar G, Hebert P . DNA barcoding largely supports 250 years of classical taxonomy: identifications for Central European bees (Hymenoptera, Apoidea partim). Mol Ecol Resour. 2015; 15(4):985-1000. DOI: 10.1111/1755-0998.12363. View

Hebert P, Ratnasingham S, deWaard J . Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. Proc Biol Sci. 2003; 270 Suppl 1:S96-9. PMC: 1698023. DOI: 10.1098/rsbl.2003.0025. View

Saitou N, Nei M . The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987; 4(4):406-25. DOI: 10.1093/oxfordjournals.molbev.a040454. View

Staats M, Arulandhu A, Gravendeel B, Holst-Jensen A, Scholtens I, Peelen T . Advances in DNA metabarcoding for food and wildlife forensic species identification. Anal Bioanal Chem. 2016; 408(17):4615-30. PMC: 4909793. DOI: 10.1007/s00216-016-9595-8. View

Zhou X, Adamowicz S, Jacobus L, DeWalt R, Hebert P . Towards a comprehensive barcode library for arctic life - Ephemeroptera, Plecoptera, and Trichoptera of Churchill, Manitoba, Canada. Front Zool. 2009; 6:30. PMC: 2800108. DOI: 10.1186/1742-9994-6-30. View

Taberlet P, Coissac E, Pompanon F, Brochmann C, Willerslev E . Towards next-generation biodiversity assessment using DNA metabarcoding. Mol Ecol. 2012; 21(8):2045-50. DOI: 10.1111/j.1365-294X.2012.05470.x. View

Kekkonen M, Mutanen M, Kaila L, Nieminen M, Hebert P . Delineating species with DNA barcodes: a case of taxon dependent method performance in moths. PLoS One. 2015; 10(4):e0122481. PMC: 4406103. DOI: 10.1371/journal.pone.0122481. View

Jordal B, Kambestad M . DNA barcoding of bark and ambrosia beetles reveals excessive NUMTs and consistent east-west divergence across Palearctic forests. Mol Ecol Resour. 2013; 14(1):7-17. DOI: 10.1111/1755-0998.12150. View

10.

Raupach M, Radulovici A . Looking back on a decade of barcoding crustaceans. Zookeys. 2016; (539):53-81. PMC: 4714055. DOI: 10.3897/zookeys.539.6530. View

11.

Raupach M, Hannig K, Moriniere J, Hendrich L . A DNA barcode library for ground beetles (Insecta, Coleoptera, Carabidae) of Germany: The genus Bembidion Latreille, 1802 and allied taxa. Zookeys. 2016; (592):121-41. PMC: 4926639. DOI: 10.3897/zookeys.592.8316. View

12.

Morgulis A, Coulouris G, Raytselis Y, Madden T, Agarwala R, Schaffer A . Database indexing for production MegaBLAST searches. Bioinformatics. 2008; 24(16):1757-64. PMC: 2696921. DOI: 10.1093/bioinformatics/btn322. View

13.

candek K, Kuntner M . DNA barcoding gap: reliable species identification over morphological and geographical scales. Mol Ecol Resour. 2014; 15(2):268-77. DOI: 10.1111/1755-0998.12304. View

14.

Smith M, Bertrand C, Crosby K, Eveleigh E, Fernandez-Triana J, Fisher B . Wolbachia and DNA barcoding insects: patterns, potential, and problems. PLoS One. 2012; 7(5):e36514. PMC: 3342236. DOI: 10.1371/journal.pone.0036514. View

15.

Scheffers B, Joppa L, Pimm S, Laurance W . What we know and don't know about Earth's missing biodiversity. Trends Ecol Evol. 2012; 27(9):501-10. DOI: 10.1016/j.tree.2012.05.008. View

16.

Werren J, Baldo L, Clark M . Wolbachia: master manipulators of invertebrate biology. Nat Rev Microbiol. 2008; 6(10):741-51. DOI: 10.1038/nrmicro1969. View

17.

Hausmann A, Godfray H, Huemer P, Mutanen M, Rougerie R, van Nieukerken E . Genetic patterns in European geometrid moths revealed by the Barcode Index Number (BIN) system. PLoS One. 2013; 8(12):e84518. PMC: 3866169. DOI: 10.1371/journal.pone.0084518. View

18.

Hajibabaei M, Janzen D, Burns J, Hallwachs W, Hebert P . DNA barcodes distinguish species of tropical Lepidoptera. Proc Natl Acad Sci U S A. 2006; 103(4):968-71. PMC: 1327734. DOI: 10.1073/pnas.0510466103. View

19.

Smith M, Fernandez-Triana J, Eveleigh E, Gomez J, Guclu C, Hallwachs W . DNA barcoding and the taxonomy of Microgastrinae wasps (Hymenoptera, Braconidae): impacts after 8 years and nearly 20 000 sequences. Mol Ecol Resour. 2012; 13(2):168-76. DOI: 10.1111/1755-0998.12038. View

20.

Kress W, Garcia-Robledo C, Uriarte M, Erickson D . DNA barcodes for ecology, evolution, and conservation. Trends Ecol Evol. 2014; 30(1):25-35. DOI: 10.1016/j.tree.2014.10.008. View