Sugarcane Mosaic Virus-Based Gene Silencing in

Overview

Journal Iran J Biotechnol

Specialty Biotechnology

Date 2018 May 31

PMID 29845078

Citations 1

Authors

Sajed Ali

Idrees Ahmad Nasir

Muhammad Rafiq

Shahid Javed Butt

Farooq Ihsan

Abdul Qayyum Rao

Tayyab Husnain

Affiliations

Soon will be listed here.

Abstract

Potyvirus-based virus-induced gene silencing (VIGS) is used for knocking down the expression of a target gene in numerous plant species. virus (SCMV) is a monopartite, positive single strand RNA virus. pBINTRA6 vector was modifi ed by inserting a gene segment of SCMV in place of (TRV) genome part 1 (TRV1 or RNA1) and the two nonstructural proteins of TRV2(RNA2). : SCMV construct was inoculated into 3-4 weeks plant leaves either by using a needleless syringe or applying pricking with a toothpick. The construct (SCMV-RNA2) successfully induced post-transcriptional gene silencing (PTGS) of the target genes and I through agroinoculation proving that SCMV is a substitute of the RNA1, which plays a pivotal role in the systemic gene silencing. 2-3-weeks of post inoculation, target genes' silencing was observed in the newly developed noninoculated leaves. The newly developed construct expresses the knocked down of the endogenous as well as exogenous genes and only four weeks are required for the transient expression of the gene silencing based on SCMV-VIGS system.

Citing Articles

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