Manipulation of Intracellular Magnesium Content in Polymyxin B Nonapeptide-sensitized Escherichia Coli by Ionophore A23187

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1985 Apr 1

PMID 2984182

Citations 88

Authors

T Alatossava

H Jutte

A Kuhn

E Kellenberger

Affiliations

Soon will be listed here.

Abstract

Escherichia coli B cells were sensitized to ionophore A23187 by polymyxin B nonapeptide, and the induced magnesium and potassium ion fluxes were studied. Combined ionophore treatment permeabilized the cytoplasmic membrane of E. coli in an ion-specific manner and allowed the manipulation of intracellular Mg2+ content from the outside. A23187-induced Mg2+ efflux or influx was dependent on the free Mg2+ concentration gradient between the outside and inside of the cytoplasmic membrane and on the pH gradient. Most of the intracellular Mg2+ was bound, whereas only 1 to 2 mM was free in solution in the cellular sap.

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