Physcomitrella Patens, a Versatile Synthetic Biology Chassis

Overview

Journal Plant Cell Rep

Publisher Springer

Date 2018 May 26

PMID 29797047

Citations 28

Authors

Ralf Reski

Hansol Bae

Henrik Toft Simonsen

Affiliations

Soon will be listed here.

Abstract

During three decades the moss Physcomitrella patens has been developed to a superb green cell factory with the first commercial products on the market. In the past three decades the moss P. patens has been developed from an obscure bryophyte to a model organism in basic biology, biotechnology, and synthetic biology. Some of the key features of this system include a wide range of Omics technologies, precise genome-engineering via homologous recombination with yeast-like efficiency, a certified good-manufacturing-practice production in bioreactors, successful upscaling to 500 L wave reactors, excellent homogeneity of protein products, superb product stability from batch-to-batch, and a reliable procedure for cryopreservation of cell lines in a master cell bank. About a dozen human proteins are being produced in P. patens as potential biopharmaceuticals, some of them are not only similar to their animal-produced counterparts, but are real biobetters with superior performance. A moss-made pharmaceutical successfully passed phase 1 clinical trials, a fragrant moss, and a cosmetic moss-product is already on the market, highlighting the economic potential of this synthetic biology chassis. Here, we focus on the features of mosses as versatile cell factories for synthetic biology and their impact on metabolic engineering.

Citing Articles

Synthetic biology and artificial intelligence in crop improvement.

Zhang D, Xu F, Wang F, Le L, Pu L Plant Commun. 2024; 6(2):101220.

PMID: 39668563 PMC: 11897457. DOI: 10.1016/j.xplc.2024.101220.

Differential prolyl hydroxylation by six Physcomitrella prolyl-4 hydroxylases.

Rempfer C, Hoernstein S, van Gessel N, Graf A, Spiegelhalder R, Bertolini A Comput Struct Biotechnol J. 2024; 23:2580-2594.

PMID: 39021582 PMC: 11252719. DOI: 10.1016/j.csbj.2024.06.014.

PpFab: An efficient promoter toolkit in Physcomitrium Patens.

Luo G, Ye H, Xu M, Li X, Zhu J, Dai J Plant Physiol. 2024; 196(1):2-6.

PMID: 38865446 PMC: 11376402. DOI: 10.1093/plphys/kiae332.

Engineering Plant Cell Fates and Functions for Agriculture and Industry.

Tansley C, Patron N, Guiziou S ACS Synth Biol. 2024; 13(4):998-1005.

PMID: 38573786 PMC: 11036505. DOI: 10.1021/acssynbio.4c00047.

Near telomere-to-telomere genome of the model plant Physcomitrium patens.

Bi G, Zhao S, Yao J, Wang H, Zhao M, Sun Y Nat Plants. 2024; 10(2):327-343.

PMID: 38278953 DOI: 10.1038/s41477-023-01614-7.

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