The β3-integrin Endothelial Adhesome Regulates Microtubule-dependent Cell Migration

Overview

Journal EMBO Rep

Specialty Molecular Biology

Date 2018 May 26

PMID 29794156

Citations 13

Authors

Samuel J Atkinson

Aleksander M Gontarczyk

Abdullah Aa Alghamdi

Tim S Ellison

Robert T Johnson

Wesley J Fowler

Benjamin M Kirkup

Bernardo C Silva

Bronwen E Harry

Jochen G Schneider

Katherine N Weilbaecher

Mette M Mogensen

Mark D Bass

Maddy Parsons

Dylan R Edwards

Stephen D Robinson

Affiliations

Soon will be listed here.

Abstract

Integrin β3 is seen as a key anti-angiogenic target for cancer treatment due to its expression on neovasculature, but the role it plays in the process is complex; whether it is pro- or anti-angiogenic depends on the context in which it is expressed. To understand precisely β3's role in regulating integrin adhesion complexes in endothelial cells, we characterised, by mass spectrometry, the β3-dependent adhesome. We show that depletion of β3-integrin in this cell type leads to changes in microtubule behaviour that control cell migration. β3-integrin regulates microtubule stability in endothelial cells through Rcc2/Anxa2-driven control of active Rac1 localisation. Our findings reveal that angiogenic processes, both and , are more sensitive to microtubule targeting agents when β3-integrin levels are reduced.

Citing Articles

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