Cigarette Smoke Promotes HIV Infection of Primary Bronchial Epithelium and Additively Suppresses CFTR Function

Overview

Journal Sci Rep

Specialty Science

Date 2018 May 24

PMID 29789655

Citations 21

Authors

S Chinnapaiyan

R Dutta

J Bala

T Parira

M Agudelo

M Nair

H J Unwalla

Affiliations

Soon will be listed here.

Abstract

Recurrent lung infections are a common cause of morbidity and mortality in people living with HIV and this is exacerbated in smokers even when administered combination antiretroviral therapy (cART). The incidence of pneumonia is increased with smoking and treatment interruption and is directly dependent on viral load in patients when adjusted for CD4 counts. CFTR dysfunction plays an important role in aberrant airway innate immunity as it is pivotal in regulating mucociliary clearance (MCC) rates and other antibacterial mechanisms of the airway. In our earlier work, we have demonstrated that bronchial epithelium expresses canonical HIV receptors CD4, CCR5 and CXCR4 and can be infected with HIV. HIV Tat suppresses CFTR mRNA and function via TGF-β signaling. In the present study, we demonstrate that cigarette smoke (CS) potentiates HIV infection of bronchial epithelial cells by upregulating CD4 and CCR5 expression. HIV and CS individually and additively suppress CFTR biogenesis and function, possibly explaining the increased incidence of lung infections in HIV patients and its exacerbation in HIV smokers.

Citing Articles

HIV-1 Tat Protein and Cigarette Smoke Mediated ADAM17 Upregulation Can Lead to Impaired Mucociliary Clearance.

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References

Wang F, He B . CCR1 and CCR5 expression on inflammatory cells is related to cigarette smoking and chronic obstructive pulmonary disease severity. Chin Med J (Engl). 2012; 125(23):4277-82. View

Rao P, Ande A, Sinha N, Kumar A, Kumar S . Effects of Cigarette Smoke Condensate on Oxidative Stress, Apoptotic Cell Death, and HIV Replication in Human Monocytic Cells. PLoS One. 2016; 11(5):e0155791. PMC: 4874604. DOI: 10.1371/journal.pone.0155791. View

Sato K, Kawasaki H, Nagayama H, Enomoto M, Morimoto C, Tadokoro K . TGF-beta 1 reciprocally controls chemotaxis of human peripheral blood monocyte-derived dendritic cells via chemokine receptors. J Immunol. 2000; 164(5):2285-95. DOI: 10.4049/jimmunol.164.5.2285. View

Chinnapaiyan S, Parira T, Dutta R, Agudelo M, Morris A, Nair M . HIV Infects Bronchial Epithelium and Suppresses Components of the Mucociliary Clearance Apparatus. PLoS One. 2017; 12(1):e0169161. PMC: 5217953. DOI: 10.1371/journal.pone.0169161. View

Bracke K, Dhulst A, Maes T, Demedts I, Moerloose K, Kuziel W . Cigarette smoke-induced pulmonary inflammation, but not airway remodelling, is attenuated in chemokine receptor 5-deficient mice. Clin Exp Allergy. 2007; 37(10):1467-79. DOI: 10.1111/j.1365-2222.2007.02808.x. View

Sethi S, Murphy T . Bacterial infection in chronic obstructive pulmonary disease in 2000: a state-of-the-art review. Clin Microbiol Rev. 2001; 14(2):336-63. PMC: 88978. DOI: 10.1128/CMR.14.2.336-363.2001. View

Wang R, Wright J, Churg A . Transforming growth factor-beta1 drives airway remodeling in cigarette smoke-exposed tracheal explants. Am J Respir Cell Mol Biol. 2005; 33(4):387-93. DOI: 10.1165/rcmb.2005-0203OC. View

Chinnapaiyan S, Unwalla H . Mucociliary dysfunction in HIV and smoked substance abuse. Front Microbiol. 2015; 6:1052. PMC: 4604303. DOI: 10.3389/fmicb.2015.01052. View

Cloonan S, Lam H, Ryter S, Choi A . "Ciliophagy": The consumption of cilia components by autophagy. Autophagy. 2014; 10(3):532-4. PMC: 4077895. DOI: 10.4161/auto.27641. View

10.

Wanner A, Salathe M, ORiordan T . Mucociliary clearance in the airways. Am J Respir Crit Care Med. 1996; 154(6 Pt 1):1868-902. DOI: 10.1164/ajrccm.154.6.8970383. View

11.

Morris A, George M, Crothers K, Huang L, Lucht L, Kessinger C . HIV and chronic obstructive pulmonary disease: is it worse and why?. Proc Am Thorac Soc. 2011; 8(3):320-5. PMC: 3132792. DOI: 10.1513/pats.201006-045WR. View

12.

Fichtenbaum C, Woeltje K, Powderly W . Serious Pseudomonas aeruginosa infections in patients infected with human immunodeficiency virus: a case-control study. Clin Infect Dis. 1994; 19(3):417-22. DOI: 10.1093/clinids/19.3.417. View

13.

Parira T, Figueroa G, Laverde A, Casteleiro G, Gomez Hernandez M, Fernandez-Lima F . Novel detection of post-translational modifications in human monocyte-derived dendritic cells after chronic alcohol exposure: Role of inflammation regulator H4K12ac. Sci Rep. 2017; 7(1):11236. PMC: 5593989. DOI: 10.1038/s41598-017-11172-6. View

14.

Clunes L, Davies C, Coakley R, Aleksandrov A, Henderson A, Zeman K . Cigarette smoke exposure induces CFTR internalization and insolubility, leading to airway surface liquid dehydration. FASEB J. 2011; 26(2):533-45. PMC: 3290447. DOI: 10.1096/fj.11-192377. View

15.

Crothers K, Huang L, Goulet J, Goetz M, Brown S, Rodriguez-Barradas M . HIV infection and risk for incident pulmonary diseases in the combination antiretroviral therapy era. Am J Respir Crit Care Med. 2010; 183(3):388-95. PMC: 3266024. DOI: 10.1164/rccm.201006-0836OC. View

16.

Hirschtick R, Glassroth J, Jordan M, Wilcosky T, Wallace J, Kvale P . Bacterial pneumonia in persons infected with the human immunodeficiency virus. Pulmonary Complications of HIV Infection Study Group. N Engl J Med. 1995; 333(13):845-51. DOI: 10.1056/NEJM199509283331305. View

17.

Fulcher M, Gabriel S, Burns K, Yankaskas J, Randell S . Well-differentiated human airway epithelial cell cultures. Methods Mol Med. 2004; 107:183-206. DOI: 10.1385/1-59259-861-7:183. View

18.

Ande A, McArthur C, Ayuk L, Awasom C, Achu P, Njinda A . Effect of mild-to-moderate smoking on viral load, cytokines, oxidative stress, and cytochrome P450 enzymes in HIV-infected individuals. PLoS One. 2015; 10(4):e0122402. PMC: 4399877. DOI: 10.1371/journal.pone.0122402. View

19.

Churg A, Tai H, Coulthard T, Wang R, Wright J . Cigarette smoke drives small airway remodeling by induction of growth factors in the airway wall. Am J Respir Crit Care Med. 2006; 174(12):1327-34. DOI: 10.1164/rccm.200605-585OC. View

20.

Guo L, Zhang Y, Zhang L, Huang F, Li J, Wang S . MicroRNAs, TGF-β signaling, and the inflammatory microenvironment in cancer. Tumour Biol. 2015; 37(1):115-25. PMC: 4841843. DOI: 10.1007/s13277-015-4374-2. View