Determination of Red Blood Cell Deformability Using Centrifugal Force in a Three-dimensional-printed Mini-disk (3D-PMD)

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2018 May 23

PMID 29787582

Citations 4

Authors

Hyunjung Lim

Seung Min Back

Jeonghun Nam

Hyuk Choi

Affiliations

Soon will be listed here.

Abstract

Measuring red blood cell (RBC) deformability has become important for clinical disease diagnostics. Various methods for measuring RBC deformability have been developed; however, they require costly and large instruments, long measuring time, and skilled personnel. In this study, we present a three-dimensional-printed mini-disk (3D-PMD) for measuring RBC deformability to overcome the previous limitations. For a miniaturized and low-cost setup, the 3D-PMD was fabricated by a 3D printing technique, which had not yet been used for fabricating a lab-on-a-compact disk (LOCD). Using a 3D printing technique, a multi-layered fluidic channel on the mini CD could be fabricated easily. During rotation by a spinning motor, the difference of the length of compressed RBCs in the fluidic channel was measured and analysed as compressibility indices (CIs) of normal and glutaraldehyde-treated hardened RBCs. The rotation speed and time were decided as 3000 rpm and 30 min, respectively, at which the difference of CI values between normal and hardened RBCs was largest (CInormal-CIhardened = 0.195).

Citing Articles

Advances in Microfluidics for Single Red Blood Cell Analysis.

Grigorev G, Lebedev A, Wang X, Qian X, Maksimov G, Lin L Biosensors (Basel). 2023; 13(1).

PMID: 36671952 PMC: 9856164. DOI: 10.3390/bios13010117.

Characterizing bulk rigidity of rigid red blood cell populations in sickle-cell disease patients.

Gutierrez M, Shamoun M, Seu K, Tanski T, Kalfa T, Eniola-Adefeso O Sci Rep. 2021; 11(1):7909.

PMID: 33846383 PMC: 8041827. DOI: 10.1038/s41598-021-86582-8.

Editorial: Red Blood Cell Vascular Adhesion and Deformability.

Guizouarn H, Barshtein G Front Physiol. 2020; 11:657.

PMID: 32670087 PMC: 7331698. DOI: 10.3389/fphys.2020.00657.

An experimental erythrocyte rigidity index (Ri) and its correlations with Transcranial Doppler velocities (TAMMV), Gosling Pulsatility Index PI, hematocrit, hemoglobin concentration and red cell distribution width (RDW).

Cardoso A PLoS One. 2020; 15(2):e0229105.

PMID: 32084188 PMC: 7034921. DOI: 10.1371/journal.pone.0229105.

References

Bennett-Guerrero E, Veldman T, Doctor A, Telen M, Ortel T, Reid T . Evolution of adverse changes in stored RBCs. Proc Natl Acad Sci U S A. 2007; 104(43):17063-8. PMC: 2040393. DOI: 10.1073/pnas.0708160104. View

Suresh S . Biomechanics and biophysics of cancer cells. Acta Biomater. 2007; 3(4):413-38. PMC: 2917191. DOI: 10.1016/j.actbio.2007.04.002. View

Guo Q, Duffy S, Matthews K, Santoso A, Scott M, Ma H . Microfluidic analysis of red blood cell deformability. J Biomech. 2014; 47(8):1767-76. DOI: 10.1016/j.jbiomech.2014.03.038. View

Huang S, Undisz A, Diez-Silva M, Bow H, Dao M, Han J . Dynamic deformability of Plasmodium falciparum-infected erythrocytes exposed to artesunate in vitro. Integr Biol (Camb). 2012; 5(2):414-22. PMC: 3615419. DOI: 10.1039/c2ib20161e. View

Biesiada G, Krzemien J, Czepiel J, Teleglow A, Dabrowski Z, Spodaryk K . Rheological properties of erythrocytes in patients suffering from erysipelas. Examination with LORCA device. Clin Hemorheol Microcirc. 2006; 34(3):383-90. View

Bow H, Pivkin I, Diez-Silva M, Goldfless S, Dao M, Niles J . A microfabricated deformability-based flow cytometer with application to malaria. Lab Chip. 2011; 11(6):1065-73. PMC: 3366288. DOI: 10.1039/c0lc00472c. View

Kong L, Perebikovsky A, Moebius J, Kulinsky L, Madou M . Lab-on-a-CD: A Fully Integrated Molecular Diagnostic System. J Lab Autom. 2015; 21(3):323-55. DOI: 10.1177/2211068215588456. View

Badr I, Johnson R, Madou M, Bachas L . Fluorescent ion-selective optode membranes incorporated onto a centrifugal microfluidics platform. Anal Chem. 2002; 74(21):5569-75. DOI: 10.1021/ac025690z. View

Alapan Y, Little J, Gurkan U . Heterogeneous red blood cell adhesion and deformability in sickle cell disease. Sci Rep. 2014; 4:7173. PMC: 4241514. DOI: 10.1038/srep07173. View

10.

Cha S, Shin T, Lee S, Shim W, Lee G, Lee S . Cell stretching measurement utilizing viscoelastic particle focusing. Anal Chem. 2012; 84(23):10471-7. DOI: 10.1021/ac302763n. View

11.

Johnson R . Ektacytometry of red blood cells. Methods Enzymol. 1989; 173:35-54. DOI: 10.1016/s0076-6879(89)73004-4. View

12.

Au A, Huynh W, Horowitz L, Folch A . 3D-Printed Microfluidics. Angew Chem Int Ed Engl. 2016; 55(12):3862-81. PMC: 7679199. DOI: 10.1002/anie.201504382. View

13.

Kar S, Dash M, Maiti T, Chakraborty S . Effect of hematocrit on blood dynamics on a compact disc platform. Analyst. 2015; 140(5):1432-7. DOI: 10.1039/c4an02020k. View

14.

Hardeman M, Besselink G, Ebbing I, de Korte D, Ince C, Verhoeven A . Laser-assisted optical rotational cell analyzer measurements reveal early changes in human RBC deformability induced by photodynamic treatment. Transfusion. 2003; 43(11):1533-7. DOI: 10.1046/j.1537-2995.2003.00566.x. View

15.

Ho C, Ng S, Li K, Yoon Y . 3D printed microfluidics for biological applications. Lab Chip. 2015; 15(18):3627-37. DOI: 10.1039/c5lc00685f. View

16.

Forsyth A, Wan J, Ristenpart W, Stone H . The dynamic behavior of chemically "stiffened" red blood cells in microchannel flows. Microvasc Res. 2010; 80(1):37-43. DOI: 10.1016/j.mvr.2010.03.008. View

17.

Chen X, Feng L, Jin H, Feng S, Yu Y . Quantification of the erythrocyte deformability using atomic force microscopy: correlation study of the erythrocyte deformability with atomic force microscopy and hemorheology. Clin Hemorheol Microcirc. 2009; 43(3):243-51. DOI: 10.3233/CH-2009-1234. View

18.

Cluitmans J, Chokkalingam V, Janssen A, Brock R, Huck W, Bosman G . Alterations in red blood cell deformability during storage: a microfluidic approach. Biomed Res Int. 2014; 2014:764268. PMC: 4176636. DOI: 10.1155/2014/764268. View

19.

Wiewiora M, Maciej W, Sosada K, Krystyn S, Slowinska L, Piecuch J . Sex-dependent differences in rheological properties and the relation of blood viscosity to erythrocyte aggregation indices among morbidly obese patients. Clin Hemorheol Microcirc. 2010; 44(4):259-67. DOI: 10.3233/CH-2010-1275. View

20.

Li X, Yu Z, Geraldo D, Weng S, Alve N, Dun W . Desktop aligner for fabrication of multilayer microfluidic devices. Rev Sci Instrum. 2015; 86(7):075008. PMC: 4522017. DOI: 10.1063/1.4927197. View