Hyperbaric Oxygen Inhibits Production of CD3+ T Cells in the Thymus and Facilitates Malignant Glioma Cell Growth

Overview

Journal J Int Med Res

Publisher Sage Publications

Specialty General Medicine

Date 2018 May 23

PMID 29785863

Citations 7

Authors

Yong-Gang Wang

Jiang Long

Dong-Chuan Shao

Hai Song

Affiliations

Soon will be listed here.

Abstract

Objective Hyperbaric oxygen (HBO) is an emerging complementary alternative medical approach in glioma treatment. However, its mode of action is unknown, so this was investigated in the present study. Methods We constructed an intracranial glioma model of congenic C57BL/6J mice. Glioma growth under HBO stimulation was assessed by bioluminescent imaging and magnetic resonance imaging. Flow cytometry assessed direct effects of HBO on reactive oxygen species (ROS) signaling of transplanted glioma cells and organs, and quantified mature T cells and subgroups in tumors, the brain, and blood. Results HBO promoted the growth of transplanted GL261-Luc glioma in the intracranial glioma mouse model. ROS signaling of glioma cells and brain cells was significantly downregulated under HBO stimulation, but thymus ROS levels were significantly upregulated. CD3+ T cells were significantly downregulated, while both Ti/Th cells (CD3+CD4+) and Ts/Tc cells (CD3+CD8+) were inhibited in tumors of the HBO group. The percentage of regulatory T cells in Ti/Th (CD3+CD4+) cells was elevated in the tumors and thymuses of the HBO group. Conclusion HBO induced ROS signaling in the thymus, inhibited CD3+ T cell generation, and facilitated malignant glioma cell growth in vivo in the intracranial glioma mouse model.

Citing Articles

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References

Ogawa K, Ishiuchi S, Inoue O, Yoshii Y, Saito A, Watanabe T . Phase II trial of radiotherapy after hyperbaric oxygenation with multiagent chemotherapy (procarbazine, nimustine, and vincristine) for high-grade gliomas: long-term results. Int J Radiat Oncol Biol Phys. 2011; 82(2):732-8. DOI: 10.1016/j.ijrobp.2010.12.070. View

Conconi M, Baiguera S, Guidolin D, Furlan C, Menti A, Vigolo S . Effects of hyperbaric oxygen on proliferative and apoptotic activities and reactive oxygen species generation in mouse fibroblast 3T3/J2 cell line. J Investig Med. 2003; 51(4):227-32. DOI: 10.1136/jim-51-04-24. View

Deb P, Boruah D, Dutta V . Morphometric study of microvessels in primary CNS tumors and its correlation with tumor types and grade. Microvasc Res. 2012; 84(1):34-43. DOI: 10.1016/j.mvr.2012.03.004. View

Kohshi K, Yamamoto H, Nakahara A, Katoh T, Takagi M . Fractionated stereotactic radiotherapy using gamma unit after hyperbaric oxygenation on recurrent high-grade gliomas. J Neurooncol. 2006; 82(3):297-303. DOI: 10.1007/s11060-006-9283-1. View

Sabel M, Giese A . Safety profile of carmustine wafers in malignant glioma: a review of controlled trials and a decade of clinical experience. Curr Med Res Opin. 2008; 24(11):3239-57. DOI: 10.1185/03007990802508180. View

Schonmeyr B, Wong A, Reid V, Gewalli F, Mehrara B . The effect of hyperbaric oxygen treatment on squamous cell cancer growth and tumor hypoxia. Ann Plast Surg. 2008; 60(1):81-8. DOI: 10.1097/SAP.0b013e31804a806a. View

Morgan L . The epidemiology of glioma in adults: a "state of the science" review. Neuro Oncol. 2015; 17(4):623-4. PMC: 4483082. DOI: 10.1093/neuonc/nou358. View

Buhler H, Strohm G, Nguemgo-Kouam P, Lamm H, Fakhrian K, Adamietz I . The therapeutic effect of photon irradiation on viable glioblastoma cells is reinforced by hyperbaric oxygen. Anticancer Res. 2015; 35(4):1977-83. View

Shin H, Iwasaki A . Tissue-resident memory T cells. Immunol Rev. 2013; 255(1):165-81. PMC: 3748618. DOI: 10.1111/imr.12087. View

10.

Biollaz G, Bernasconi L, Cretton C, Puntener U, Frei K, Fontana A . Site-specific anti-tumor immunity: differences in DC function, TGF-beta production and numbers of intratumoral Foxp3+ Treg. Eur J Immunol. 2009; 39(5):1323-33. DOI: 10.1002/eji.200838921. View

11.

Sathornsumetee S, Cao Y, Marcello J, Herndon 2nd J, McLendon R, Desjardins A . Tumor angiogenic and hypoxic profiles predict radiographic response and survival in malignant astrocytoma patients treated with bevacizumab and irinotecan. J Clin Oncol. 2008; 26(2):271-8. PMC: 3930173. DOI: 10.1200/JCO.2007.13.3652. View

12.

Kingwell K . Neuro-oncology: Glioblastoma prognosis linked to neuronal PD-L1 expression in tumour-adjacent tissue. Nat Rev Neurol. 2013; 9(11):602-3. DOI: 10.1038/nrneurol.2013.197. View

13.

Myung J, Cho H, Kim H, Park C, Lee S, Choi S . Prognosis of Glioblastoma With Oligodendroglioma Component is Associated With the IDH1 Mutation and MGMT Methylation Status. Transl Oncol. 2014; 7(6):712-9. PMC: 4311043. DOI: 10.1016/j.tranon.2014.10.002. View

14.

Murphy M, Siegel R . Mitochondrial ROS fire up T cell activation. Immunity. 2013; 38(2):201-2. DOI: 10.1016/j.immuni.2013.02.005. View

15.

See A, Parker J, Waziri A . The role of regulatory T cells and microglia in glioblastoma-associated immunosuppression. J Neurooncol. 2015; 123(3):405-12. DOI: 10.1007/s11060-015-1849-3. View

16.

Dagistan Y, Karaca I, Bozkurt E, Ozar E, Yagmurlu K, Toklu A . Combination hyperbaric oxygen and temozolomide therapy in C6 rat glioma model. Acta Cir Bras. 2012; 27(6):383-7. DOI: 10.1590/s0102-86502012000600005. View

17.

Lu X, Cao K, Li Q, Yuan Z, Lu P . The synergistic therapeutic effect of temozolomide and hyperbaric oxygen on glioma U251 cell lines is accompanied by alterations in vascular endothelial growth factor and multidrug resistance-associated protein-1 levels. J Int Med Res. 2012; 40(3):995-1004. DOI: 10.1177/147323001204000318. View

18.

Calabrese C, Poppleton H, Kocak M, Hogg T, Fuller C, Hamner B . A perivascular niche for brain tumor stem cells. Cancer Cell. 2007; 11(1):69-82. DOI: 10.1016/j.ccr.2006.11.020. View

19.

Zhou Y, Zhou Y, Shingu T, Feng L, Chen Z, Ogasawara M . Metabolic alterations in highly tumorigenic glioblastoma cells: preference for hypoxia and high dependency on glycolysis. J Biol Chem. 2011; 286(37):32843-53. PMC: 3173179. DOI: 10.1074/jbc.M111.260935. View

20.

Sun S, Lee D, Lee N, Pu J, Wong S, Lui W . Hyperoxia resensitizes chemoresistant human glioblastoma cells to temozolomide. J Neurooncol. 2012; 109(3):467-75. PMC: 3434886. DOI: 10.1007/s11060-012-0923-3. View