Enhanced Suppressive Activity of Regulatory T Cells in the Microenvironment of Malignant Pleural Effusions

Overview

Journal J Immunol Res

Publisher Wiley

Specialty Allergy & Immunology

Date 2018 May 23

PMID 29785404

Citations 9

Authors

Joanna Budna

Mariusz Kaczmarek

Agata Kolecka-Bednarczyk

Lukasz Spychalski

Piotr Zawierucha

Joanna Gozdzik-Spychalska

Michal Nowicki

Halina Batura-Gabryel

Jan Sikora

Affiliations

Soon will be listed here.

Abstract

Cancer metastatic spread to serous cavity causes malignant pleural effusions (MPEs), indicating dismal prognosis. Tumor microenvironment can implement suppressive activity on host immune responses. Thus, we investigated the prevalence of Tregs and the relationship between them and TGF- and IL-10 concentrations and measured expression of , , , and genes, as well as protein expression of selected genes in benign effusions and MPEs. The percentage of Tregs was determined by means of multicolor flow cytometry system. TGF- and IL-10 concentrations were measured using human TGF-1 and IL-10 ELISA kit. Relative mRNA expression of studied genes was analyzed by real-time PCR. The frequency of Tregs was significantly higher in MPEs compared to benign effusions; however, the level of TGF- and IL-10 in analyzed groups was comparable, and no correlation between concentrations of TGF- and IL-10 and percentage of Tregs was observed. Relative mRNA expression of all the genes was higher in CD4CD25 compared to CD4CD25 cells. In CD4CD25 cells from MPEs, relative mRNA expression of , , and genes was significantly higher than in benign effusions; however, the level of CD4CD25CTLA-4 cells in analyzed groups showed no significant differences. We found numerous genes correlations in an entire CD4CD25 cell subset and CD4CD25 cells from MPEs. Enhanced suppressive activity of Tregs is observed in the microenvironment of MPEs. Understanding of relations between cellular and cytokine immunosuppressive factors in tumor microenvironment may determine success of anticancer response.

Citing Articles

Interleukin-8/CXCR1 Signaling Contributes to the Progression of Pulmonary Adenocarcinoma Resulting in Malignant Pleural Effusion.

Chang Y, Huang W, Yang S, Jan C, Nieh S, Lin Y Cells. 2024; 13(11.

PMID: 38891100 PMC: 11172099. DOI: 10.3390/cells13110968.

A maladaptive pleural environment suppresses preexisting anti-tumor activity of pleural infiltrating T cells.

Donnenberg V, Luketich J, Sultan I, Lister J, Bartlett D, Ghosh S Front Immunol. 2023; 14:1157697.

PMID: 37063842 PMC: 10097923. DOI: 10.3389/fimmu.2023.1157697.

A Case of Pneumonia Masking Pleural Malignancy.

Xu Q, Tian J, Huang L, Zhong Q, Xu Y, Liu L Onco Targets Ther. 2022; 15:741-746.

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Prognostic role of immune microenvironment in pleural metastases from breast and lung adenocarcinomas.

Karpathiou G, Benli J, Desage A, Jacob M, Tiffet O, Peoch M Ann Transl Med. 2022; 10(8):430.

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Th17 cells and their related cytokines: vital players in progression of malignant pleural effusion.

Niu Y, Zhou Q Cell Mol Life Sci. 2022; 79(4):194.

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