Assessing Methanogenic Archaeal Community in Full Scale Anaerobic Sludge Digester Systems in Dubai, United Arab Emirates

Overview

Journal Open Microbiol J

Publisher Bentham Open

Specialty Microbiology

Date 2018 May 23

PMID 29785219

Citations 2

Authors

Munawwar A Khan

Poojabahen G Patel

Arpitha G Ganesh

Naushad Rais

Sultan M Faheem

Shams T Khan

Affiliations

Soon will be listed here.

Abstract

Introduction: Anaerobic digestion for methane production comprises of an exceptionally diverse microbial consortium, a profound understanding about which is still constrained. In this study, the methanogenic archaeal communities in three full-scale anaerobic digesters of a Municipal Wastewater Treatment Plant were analyzed by Fluorescence hybridization and quantitative real-time Polymerase Chain Reaction (qPCR) technique.

Methods & Materials: Fluorescence hybridization (FISH) was performed to detect and quantify the methanogenic in the sludge samples whereas qPCR was carried out to support the FISH analysis. Multiple probes targeting domain archaea, different orders and families of Archaea were used for the studies.

Results And Discussion: In general, the aceticlastic organisms were more abundant than the hydrogenotrophic organisms . Both FISH and qPCR indicated that family was the most abundant suggesting that aceticlastic methanogenesis is probably the dominant methane production pathway in these digesters.

Conclusion: Future work involving high-throughput sequencing methods and correlating archaeal communities with the main operational parameters of anaerobic digesters will help to obtain a better understanding of the dynamics of the methanogenic archaeal community in wastewater treatment plants in United Arab Emirates (UAE) which in turn would lead to improved performance of anaerobic sludge digesters.

Citing Articles

Effects of combined tannic acid/fluoride on sulfur transformations and methanogenic pathways in swine manure.

Dalby F, Nikolausz M, Hansen M, Feilberg A PLoS One. 2021; 16(9):e0257759.

PMID: 34555107 PMC: 8459979. DOI: 10.1371/journal.pone.0257759.

Illumina sequencing of 16S rRNA genes reveals a unique microbial community in three anaerobic sludge digesters of Dubai.

Khan M, Khan S, Sequeira M, Faheem S, Rais N PLoS One. 2021; 16(4):e0249023.

PMID: 33793629 PMC: 8016227. DOI: 10.1371/journal.pone.0249023.

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