Continuous Addition of Progenitors Forms the Cardiac Ventricle in Zebrafish

Overview

Journal Nat Commun

Specialty Biology

Date 2018 May 23

PMID 29784942

Citations 34

Authors

Anastasia Felker

Karin D Prummel

Anne M Merks

Michaela Mickoleit

Eline C Brombacher

Jan Huisken

Daniela Panakova

Christian Mosimann

Affiliations

Soon will be listed here.

Abstract

The vertebrate heart develops from several progenitor lineages. After early-differentiating first heart field (FHF) progenitors form the linear heart tube, late-differentiating second heart field (SHF) progenitors extend the atrium and ventricle, and form inflow and outflow tracts (IFT/OFT). However, the position and migration of late-differentiating progenitors during heart formation remains unclear. Here, we track zebrafish heart development using transgenics based on the cardiopharyngeal gene tbx1. Live imaging uncovers a tbx1 reporter-expressing cell sheath that continuously disseminates from the lateral plate mesoderm towards the forming heart tube. High-speed imaging and optogenetic lineage tracing corroborates that the zebrafish ventricle forms through continuous addition from the undifferentiated progenitor sheath followed by late-phase accrual of the bulbus arteriosus (BA). FGF inhibition during sheath migration reduces ventricle size and abolishes BA formation, refining the window of FGF action during OFT formation. Our findings consolidate previous end-point analyses and establish zebrafish ventricle formation as a continuous process.

Citing Articles

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