Detection of Ostecalcin in Gingival Crevicular Fluid in a Group of Orthodontic Patients

Overview

Journal J Int Soc Prev Community Dent

Date 2018 May 22

PMID 29780743

Citations 1

Authors

Najwa Adeeb Nassrawin

Affiliations

Soon will be listed here.

Abstract

Aim: To detect osteocalcin (OC) in gingival crevicular fluid (GCF) and to monitor the concentration of OC at what stage inflammation and bone resorption reaches their maximum following orthodontic activation.

Materials And Methods: GCF samples were collected from six adult orthodontic patients (mean age = 22.3, range 20-24 years) on 3, 7, 10, 14, 21, 28, and 35 days after activation of orthodontic appliance, from the tooth surface where bone resorption was expected to occur. A total of 330 GCF sample were collected using filter paper strip, the volume measured by weighing. OC was analyzed using Enzyme-Linked immunoassay technique. Data were analyzed using the Statistical Package for Social Sciences software, SPSS (SPSS Inc., Chicago, IL, USA) version 15.

Results: An increase in GCF volume and flow rate was noted in the 10 day after activation of the orthodontic appliance activation; however, due to high-standard deviation, the result was not significant. OC was detected in all GCF samples. The amount and concentration were quite variable. Increase in the amount of OC was observed between days 7 and 14.

Conclusion: OC was detected in all samples. The quantity of OC increased at day 10 in a number of samples. There was no obvious association between OC concentration and time of collection.

Citing Articles

Challenges and Perspectives Regarding the Determination of Gingival Crevicular Fluid Biomarkers During Orthodontic Treatment: A Narrative Review.

Bud A, Lazar L, Martu M, Dako T, Suciu M, Vlasiu A Medicina (Kaunas). 2025; 60(12.

PMID: 39768884 PMC: 11728204. DOI: 10.3390/medicina60122004.

Dietary Alpha-Ketoglutarate Supplementation Improves Bone Growth, Phosphorus Digestion, and Growth Performance in Piglets.

Tian J, Yang F, Bao X, Jiang Q, Li Y, Yao K Animals (Basel). 2023; 13(4).

PMID: 36830356 PMC: 9951703. DOI: 10.3390/ani13040569.

References

Ingman T, Apajalahti S, Mantyla P, Savolainen P, Sorsa T . Matrix metalloproteinase-1 and -8 in gingival crevicular fluid during orthodontic tooth movement: a pilot study during 1 month of follow-up after fixed appliance activation. Eur J Orthod. 2005; 27(2):202-7. DOI: 10.1093/ejo/cjh097. View

Kunimatsu K, Mataki S, Tanaka H, Mine N, Kiyoki M, Hosoda K . A cross-sectional study on osteocalcin levels in gingival crevicular fluid from periodontal patients. J Periodontol. 1993; 64(9):865-9. DOI: 10.1902/jop.1993.64.9.865. View

Champagne C, Buchanan W, Reddy M, Preisser J, Beck J, Offenbacher S . Potential for gingival crevice fluid measures as predictors of risk for periodontal diseases. Periodontol 2000. 2003; 31:167-80. DOI: 10.1034/j.1600-0757.2003.03110.x. View

Murata M, Kato Y, Suda S, Nunokawa Y, Kobayashi Y, Takeda H . Osteocalcin, deoxypyridinoline and interleukin-1beta in peri-implant crevicular fluid of patients with peri-implantitis. Clin Oral Implants Res. 2003; 13(6):637-43. DOI: 10.1034/j.1600-0501.2002.130610.x. View

Vanderschueren D, Gevers G, Raymaekers G, Devos P, Dequeker J . Sex- and age-related changes in bone and serum osteocalcin. Calcif Tissue Int. 1990; 46(3):179-82. DOI: 10.1007/BF02555041. View

Alfaqeeh S, Anil S . Osteocalcin and N-telopeptides of type I collagen marker levels in gingival crevicular fluid during different stages of orthodontic tooth movement. Am J Orthod Dentofacial Orthop. 2011; 139(6):e553-9. DOI: 10.1016/j.ajodo.2011.03.005. View

Bullon P, Goberna B, Guerrero J, Segura J, Perez-Cano R, Martinez-Sahuquillo A . Serum, saliva, and gingival crevicular fluid osteocalcin: their relation to periodontal status and bone mineral density in postmenopausal women. J Periodontol. 2005; 76(4):513-9. DOI: 10.1902/jop.2005.76.4.513. View

Nakashima K, Roehrich N, CIMASONI G . Osteocalcin, prostaglandin E2 and alkaline phosphatase in gingival crevicular fluid: their relations to periodontal status. J Clin Periodontol. 1994; 21(5):327-33. DOI: 10.1111/j.1600-051x.1994.tb00721.x. View

AlRowis R, AlMoharib H, AlMubarak A, Bhaskardoss J, Preethanath R, Anil S . Oral fluid-based biomarkers in periodontal disease - part 2. Gingival crevicular fluid. J Int Oral Health. 2014; 6(5):126-35. PMC: 4229821. View

10.

Dudic A, Kiliaridis S, Mombelli A, Giannopoulou C . Composition changes in gingival crevicular fluid during orthodontic tooth movement: comparisons between tension and compression sides. Eur J Oral Sci. 2006; 114(5):416-22. DOI: 10.1111/j.1600-0722.2006.00387.x. View

11.

Isik F, Sayinsu K, Arun T, Unlucerci Y . Bone marker levels in gingival crevicular fluid during orthodontic intrusive tooth movement: a preliminary study. J Contemp Dent Pract. 2005; 6(2):27-35. View

12.

Mah J, Prasad N . Dentine phosphoproteins in gingival crevicular fluid during root resorption. Eur J Orthod. 2004; 26(1):25-30. DOI: 10.1093/ejo/26.1.25. View

13.

Delmas P, Glorieux F, Delvin E, Salle B, Melki I . Perinatal serum bone Gla-protein and vitamin D metabolites in preterm and fullterm neonates. J Clin Endocrinol Metab. 1987; 65(3):588-91. DOI: 10.1210/jcem-65-3-588. View

14.

Ronnerman A, Thilander B, Heyden G . Gingival tissue reactions to orthodontic closure of extraction sites. Histologic and histochemical studies. Am J Orthod. 1980; 77(6):620-5. DOI: 10.1016/0002-9416(80)90153-0. View

15.

Kumar A, Saravanan K, Kohila K, Kumar S . Biomarkers in orthodontic tooth movement. J Pharm Bioallied Sci. 2015; 7(Suppl 2):S325-30. PMC: 4606613. DOI: 10.4103/0975-7406.163437. View

16.

Lowney J, Norton L, Shafer D, Rossomando E . Orthodontic forces increase tumor necrosis factor alpha in the human gingival sulcus. Am J Orthod Dentofacial Orthop. 1995; 108(5):519-24. DOI: 10.1016/s0889-5406(95)70052-8. View

17.

Farahani M, Safavi S, Dianat O, Tusi S, Younessian F . Acid and Alkaline Phosphatase Levels in GCF during Orthodontic Tooth Movement. J Dent (Shiraz). 2015; 16(3 Suppl):237-45. PMC: 4623837. View

18.

Erdemir E, Karsiyaka Hendek M, Keceli H, Apan T . Crevicular fluid levels of interleukin-8, interleukin-17 and soluble intercellular adhesion molecule-1 after regenerative periodontal therapy. Eur J Dent. 2015; 9(1):60-65. PMC: 4319302. DOI: 10.4103/1305-7456.149644. View

19.

Vikram V, Ramakrishnan T, Anilkumar K, Ambalavanan N . Changes in Transforming Growth Factor-β1 in Gingival Crevicular Fluid of Patients with Chronic Periodontitis Following Periodontal Flap Surgery. J Clin Diagn Res. 2015; 9(2):ZC13-6. PMC: 4378799. DOI: 10.7860/JCDR/2015/11039.5539. View

20.

Oh H, Hirano J, Takai H, Ogata Y . Effects of initial periodontal therapy on interleukin-1β level in gingival crevicular fluid and clinical periodontal parameters. J Oral Sci. 2015; 57(2):67-71. DOI: 10.2334/josnusd.57.67. View