Three-way DNA Junction Based Platform For ultra-sensitive Fluorometric Detection of Multiple Metal Ions As Exemplified for Cu(II), Mg(II) and Pb(II)

Overview

Journal Mikrochim Acta

Specialties Biotechnology
Chemistry

Date 2018 May 21

PMID 29779060

Authors

Wen Yun

Xiaoqing Du

Junsheng Liao

Ge Sang

Lin Chen

Ning Li

Lizhu Yang

Affiliations

Soon will be listed here.

Abstract

A DNA-based fluorometric method is described for simultaneous determination of multiple metal ions. It is based on recycling cleavage of hairpins by using a three-way DNA junction structure. Three DNA sequences containing a binding region and an enzyme-strand (E-DNA) region are hybridized to form a three-way DNA junction. The enzyme strand regions at the end of the DNA sequence binds to the substrate sequence (S-DNA) at the loop of the hairpin to form typical DNAzyme structures. In the presence of analyte metal ions, the DNAzyme structure thus formed cleaves the loop of hairpins. This is accompanied by a release of fluorescently labeled DNA fragments and by quenching of fluorescence. The detection limits are 35 pM for Cu(II), 2 nM for Mg(II), and 8 pM for Pb(II). This method was successfully applied to the simultaneous determination of these ions in spiked human serum. Graphical abstract Schematic presentation of the recycling cleavage of hairpins by using a three-way DNA junction structure. It causes a release of fluorescently labeled DNA fragments and quenching of fluorescence. It was successfully applied to the simultaneous determination of Cu(II), Mg(II) and Pb(II) in spiked human serum.

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