DNA Methylation Signatures of Breast Cancer in Peripheral T-cells

Overview

Journal BMC Cancer

Publisher Biomed Central

Specialty Oncology

Date 2018 May 20

PMID 29776342

Citations 22

Authors

Surabhi Parashar

David Cheishvili

Niaz Mahmood

Ani Arakelian

Imrana Tanvir

Haseeb Ahmed Khan

Richard Kremer

Catalin Mihalcioiu

Moshe Szyf

Shafaat A Rabbani

Affiliations

Soon will be listed here.

Abstract

Background: Immune surveillance acts as a defense mechanism in cancer, and its disruption is involved in cancer progression. DNA methylation reflects the phenotypic identity of cells and recent data suggested that DNA methylation profiles of T cells and peripheral blood mononuclear cells (PBMC) are altered in cancer progression.

Methods: We enrolled 19 females with stage 1 and 2, nine with stage 3 and 4 and 9 age matched healthy women. T cells were isolated from peripheral blood and extracted DNA was subjected to Illumina 450 K DNA methylation array analysis. Raw data was analyzed by BMIQ, ChAMP and ComBat followed by validation of identified genes by pyrosequencing.

Results: Analysis of data revealed ~ 10,000 sites that correlated with breast cancer progression and established a list of 89 CG sites that were highly correlated (p < 0.01, r > 0.7, r < - 0.7) with breast cancer progression. The vast majority of these sites were hypomethylated and enriched in genes with functions in the immune system.

Conclusions: The study points to the possibility of using DNA methylation signatures as a noninvasive method for early detection of breast cancer and its progression which need to be tested in clinical studies.

Citing Articles

Pre-diagnosis blood DNA methylation profiling of twin pairs discordant for breast cancer points to the importance of environmental risk factors.

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PMID: 39558433 PMC: 11574988. DOI: 10.1186/s13148-024-01767-y.

Clinical validation of peripheral blood mononuclear cell DNA methylation markers for accurate early detection of hepatocellular carcinoma in Asian patients.

Cheishvili D, Wong C, Karim M, Kibria M, Jahan N, Chandra Das P Commun Med (Lond). 2024; 4(1):220.

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Methods in DNA methylation array dataset analysis: A review.

Sahoo K, Sundararajan V Comput Struct Biotechnol J. 2024; 23:2304-2325.

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Investigating FGFR2 gene as a blood-based epigenetic biomarker in gastric cancer.

Aleyasin S, Moradi A, Abolhasani N, Abdollahi M Mol Biol Rep. 2024; 51(1):253.

PMID: 38302798 DOI: 10.1007/s11033-023-09082-0.

A multiplex blood-based assay targeting DNA methylation in PBMCs enables early detection of breast cancer.

Wang T, Li P, Qi Q, Zhang S, Xie Y, Wang J Nat Commun. 2023; 14(1):4724.

PMID: 37550304 PMC: 10406825. DOI: 10.1038/s41467-023-40389-5.

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