Construction and Characterization of a Bacterial Artificial Chromosome Library for Gossypium Mustelinum

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2018 May 18

PMID 29771937

Citations 3

Authors

Yuling Liu

Baohong Zhang

Xinpeng Wen

Shulin Zhang

Yangyang Wei

Quanwei Lu

Zhen Liu

Kunbo Wang

Fang Liu

Renhai Peng

Affiliations

Soon will be listed here.

Abstract

A bacterial artificial chromosome (BAC) library for G. mustelinum Miers ex G. Watt (AD4) was constructed. Intact nuclei from G. mustelinum (AD4) were used to isolate high molecular weight DNA, which was partially cleaved with Hind III and cloned into pSMART BAC (Hind III) vectors. The BAC library consisted of 208,182 clones arrayed in 542 384-microtiter plates, with an average insert size of 121.72 kb ranging from 100 to 150 kb. About 2% of the clones did not contain inserts. Based on an estimated genome size of 2372 Mb for G. mustelinum, the BAC library was estimated to have a total coverage of 10.50 × genome equivalents. The high capacity library of G. mustelinum will serve as a giant gene resource for map-based cloning of quantitative trait loci or genes associated with important agronomic traits or resistance to Verticillium wilt, physical mapping and comparative genome analysis.

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