Suppression of External NADPH Dehydrogenase-NDB1 in Confers Improved Tolerance to Ammonium Toxicity Via Efficient Glutathione/Redox Metabolism

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2018 May 12

PMID 29747392

Citations 9

Authors

Anna Podgorska

Monika Ostaszewska-Bugajska

Klaudia Borysiuk

Agata Tarnowska

Monika Jakubiak

Maria Burian

Allan G Rasmusson

Bozena Szal

Affiliations

Soon will be listed here.

Abstract

Environmental stresses, including ammonium (NH₄⁺) nourishment, can damage key mitochondrial components through the production of surplus reactive oxygen species (ROS) in the mitochondrial electron transport chain. However, alternative electron pathways are significant for efficient reductant dissipation in mitochondria during ammonium nutrition. The aim of this study was to define the role of external NADPH-dehydrogenase (NDB1) during oxidative metabolism of NH₄⁺-fed plants. Most plant species grown with NH₄⁺ as the sole nitrogen source experience a condition known as “ammonium toxicity syndrome”. Surprisingly, transgenic plants suppressing were more resistant to NH₄⁺ treatment. The knock-down line was characterized by milder oxidative stress symptoms in plant tissues when supplied with NH₄⁺. Mitochondrial ROS accumulation, in particular, was attenuated in the knock-down plants during NH₄⁺ treatment. Enhanced antioxidant defense, primarily concerning the glutathione pool, may prevent ROS accumulation in NH₄⁺-grown -suppressing plants. We found that induction of glutathione peroxidase-like enzymes and peroxiredoxins in the -surpressing line contributed to lower ammonium-toxicity stress. The major conclusion of this study was that NDB1 suppression in plants confers tolerance to changes in redox homeostasis that occur in response to prolonged ammonium nutrition, causing cross tolerance among plants.

Citing Articles

Glyoxalase I activity affects Arabidopsis sensitivity to ammonium nutrition.

Borysiuk K, Ostaszewska-Bugajska M, Kryzheuskaya K, Gardestrom P, Szal B Plant Cell Rep. 2022; 41(12):2393-2413.

PMID: 36242617 PMC: 9700585. DOI: 10.1007/s00299-022-02931-5.

Metabolism and Signaling of Plant Mitochondria in Adaptation to Environmental Stresses.

Barreto P, Koltun A, Nonato J, Yassitepe J, Maia I, Arruda P Int J Mol Sci. 2022; 23(19).

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Quantitative trait loci mapping reveals important genomic regions controlling root architecture and shoot biomass under nitrogen, phosphorus, and potassium stress in rapeseed ( L.).

Ahmad N, Ibrahim S, Tian Z, Kuang L, Wang X, Wang H Front Plant Sci. 2022; 13:994666.

PMID: 36172562 PMC: 9511887. DOI: 10.3389/fpls.2022.994666.

Respiratory Burst Oxidase Homolog D as a Modulating Component of Oxidative Response under Ammonium Toxicity.

Burian M, Podgorska A, Ostaszewska-Bugajska M, Szal B Antioxidants (Basel). 2022; 11(4).

PMID: 35453389 PMC: 9031508. DOI: 10.3390/antiox11040703.

Nitrate alleviates ammonium toxicity in wheat ( L.) by regulating tricarboxylic acid cycle and reducing rhizospheric acidification and oxidative damage.

Du W, Zhang Y, Si J, Zhang Y, Fan S, Xia H Plant Signal Behav. 2021; 16(12):1991687.

PMID: 34753392 PMC: 9208799. DOI: 10.1080/15592324.2021.1991687.

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